Mouse Insulin-like growth factor binding protein 4,IGFBP-4 ELISA kit

Code CSB-E07355m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
insulin-like growth factor binding protein 4
Alternative Names
Igfbp4 ELISA Kit; Igfbp-4 ELISA Kit; Insulin-like growth factor-binding protein 4 ELISA Kit; IBP-4 ELISA Kit; IGF-binding protein 4 ELISA Kit; IGFBP-4 ELISA Kit
Abbreviation
IGFBP4
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma
Detection Range
0.78 ng/mL-50 ng/mL
Sensitivity
0.195 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFBP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 92    
Range % 88-96    
1:2 Average % 84    
Range % 80-88    
1:4 Average % 97    
Range % 92-101    
1:8 Average % 105    
Range % 100-112    
Recovery
The recovery of mouse IGFBP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 95 90-100    
EDTA plasma (n=4) 88 84-92    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
50 2.384 2.495 2.440 2.290    
25 2.041 2.103 2.072 1.922    
12.5 1.605 1.569 1.587 1.437    
6.25 1.002 0.981 0.992 0.842    
3.12 0.519 0.538 0.529 0.379    
1.56 0.339 0.352 0.346 0.196    
0.78 0.263 0.274 0.269 0.119    
0 0.152 0.147 0.150      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse IGFBP4 ELISA Kit was designed for the quantitative measurement of Mouse IGFBP4 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.

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Target Background

Function
(From Uniprot)
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Gene References into Functions
  1. Igfbp4 is required for inguinal fat expansion in female mice but not in male mice. However, gonadal WAT expansion, which is prevented by estrogen during high-fat diet, does not require Igfbp4. PMID: 28938423
  2. IGFBP-4 modulates the skeleton in a gender-specific manner, acting as both a cell autonomous and cell non-autonomous factor. PMID: 28184001
  3. exerts a negative effect on basal intestinal growth but plays a positive regulatory role in the intestinotropic actions of GLP-2 PMID: 25514089
  4. IGF-1/IGFbp4 signaling regulated the post-developmental adipose tissue expansion. PMID: 24778188
  5. SOX9 is a transcriptional regulator of IGFBP-4 and that SOX9-induced activation of IGFBP-4 may be one of the mechanisms by which SOX9 suppresses cell proliferation and progression of colon cancer. PMID: 23660500
  6. findings indicate that the defect in p53 function and the increased proteolysis of IGFBP-4, we had observed, represent two components of the same pathway, which contributes to the oncogenic function of Skp2B PMID: 21829624
  7. Inactivation of IGFBP4 diminished the anabolic effects of PAPP-A on bone. PMID: 20144555
  8. Targeted expression of a protease-resistant IGFBP-4 mutant in smooth muscle of transgenic mice PMID: 11923290
  9. IGFBP-4 is a potential growth inhibitor of lymphoid tissues. PMID: 15072544
  10. This study demonstrates that ES of muscle cells in vitro not only directly modulates the gene expression of contractile proteins but also modulates proteins that are part of the IGF regulatory system, in particular IGFBP-4. PMID: 15584093
  11. Crystallization, heparin and IGF binding, and osteogenic activity of IGFBP4 in its native conformation PMID: 15735305
  12. Overexpression of Hbegf in transgenic mice downregulates Igfbp4. PMID: 16019424
  13. IGFBP-4 may have a role in increasing necrosis and apoptosis, but in decreasing mitosis PMID: 17824980
  14. Results indicate that IGFBP-4 functions as a local reservoir to optimize IGF-II actions needed for normal embryogenesis, and also establish that IGFBP-4 proteolysis is required to activate most, if not all, IGF-II mediated growth-promoting activity. PMID: 18258685
  15. IGFBP-4 is an inhibitor of the canonical Wnt signalling required for cardiogenesis and provides a molecular link between insulin-like growth factor signalling and Wnt signalling PMID: 18528331
  16. SOD1(G93A) mutant transgenic mice as a model of amyotrophic lateral sclerosis (ALS) and immunohistochemical studies to investigate the changes of insulin-like growth factor-binding protein 4 (IGFBP4) in the central nervous system. PMID: 18823765

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Subcellular Location
Secreted.
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