Mouse Insulin-like peptide INSL5(INSL5) ELISA kit

Code CSB-EL011749MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
insulin-like 5
Alternative Names
Insl5 ELISA Kit; Rif ELISA Kit; Rif2 ELISA Kit; Zins3 ELISA Kit; Insulin-like peptide INSL5 ELISA Kit; Insulin-like peptide 5 ELISA Kit; Relaxin/insulin-like factor 2 ELISA Kit; Relaxin/insulin-like protein) [Cleaved into: Insulin-like peptide INSL5 B chain; Insulin-like peptide INSL5 A chain] ELISA Kit
Abbreviation
INSL5
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
3.12 pg/mL-200 pg/mL
Sensitivity
0.78 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse INSL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %93
Range %90-96
1:200Average %100
Range %97-103
1:400Average %88
Range %85-93
1:800Average %107
Range %103-110
Recovery
The recovery of mouse INSL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8481-89
EDTA plasma (n=4)9893-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
2002.417 2.446 2.432 2.262
1002.046 2.008 2.027 1.857
501.562 1.568 1.565 1.395
251.017 1.035 1.026 0.856
12.50.692 0.669 0.681 0.511
6.250.425 0.435 0.430 0.260
3.120.306 0.330 0.318 0.148
00.169 0.171 0.170
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse INSL5 ELISA Kit was designed for the quantitative measurement of Mouse INSL5 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

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Target Background

Function
(From Uniprot)
May have a role in gut contractility or in thymic development and regulation. Activates RXFP4 with high potency and appears to be the endogenous ligand for this receptor.
Gene References into Functions
  1. Mass spectral analysis of purified enteroendocrine cells and tissue homogenates identified the exact sequence of A and B chains of INSL5 endogenously expressed in L-cells. PMID: 28857318
  2. Insl5 is an orexigenic hormone released from colonic L-cells, which promotes appetite during conditions of energy deprivation. PMID: 25028498
  3. INSL5 may be a unique marker of colorectal enteroendocrine cells, and INSL5-RXFP4 signaling might play a role in an autocrine/paracrine fashion in the colorectal epithelium and rectal neuroendocrine tumors. PMID: 23438439
  4. Insl5 knockout mice displayed impaired male and female fertility that are due to marked reduction in sperm motility and irregular length of the estrous cycle. Also impaired glucose homeostasis due to glucose intolerance. PMID: 22822165
  5. Insulin-like peptide 5 is expressed in mouse brain and has a role in mobilization of calcium PMID: 16601133

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Subcellular Location
Secreted.
Protein Families
Insulin family
Tissue Specificity
Highest expression in colon with lower levels in thymus. Minimal levels in testis.
Database Links
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