Mouse L-amino-acid oxidase(IL4I1) ELISA kit

Code CSB-EL011660MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
interleukin 4 induced 1
Alternative Names
Il4i1 ELISA Kit; Fig1 ELISA Kit; L-amino-acid oxidase ELISA Kit; LAAO ELISA Kit; LAO ELISA Kit; EC 1.4.3.2 ELISA Kit; Interleukin-4-induced protein 1 ELISA Kit; IL4-induced protein 1 ELISA Kit; Protein Fig-1 ELISA Kit; mFIG1 ELISA Kit
Abbreviation
IL4I1
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL4I1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 101    
Range % 92-105    
1:2 Average % 94    
Range % 90-99    
1:4 Average % 95    
Range % 87-98    
1:8 Average % 92    
Range % 85-95    
Recovery
The recovery of mouse IL4I1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 103 94-107    
EDTA plasma (n=4) 98 90-102    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
1000 1.780 1.814 1.797 1.596    
500 1.316 1.301 1.309 1.108    
250 0.954 0.939 0.947 0.746    
125 0.716 0.732 0.724 0.523    
62.5 0.488 0.498 0.493 0.292    
31.2 0.406 0.400 0.403 0.202    
15.6 0.306 0.294 0.300 0.099    
0 0.203 0.199 0.201      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse IL4I1 ELISA Kit was designed for the quantitative measurement of Mouse IL4I1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Target Background

Function
(From Uniprot)
Secreted L-amino-acid oxidase that acts as a key immunoregulator. Has preference for L-aromatic amino acids: converts phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp) to phenylpyruvic acid (PP), hydroxyphenylpyruvic acid (HPP), and indole-3-pyruvic acid (I3P), respectively. Also has weak L-arginine oxidase activity. Acts as a negative regulator of anti-tumor immunity by mediating Trp degradation via an indole pyruvate pathway that activates the transcription factor AHR. IL4I1-mediated Trp catabolism generates I3P, giving rise to indole metabolites (indole-3-acetic acid (IAA) and indole-3-aldehyde (I3A)) and kynurenic acid, which act as ligands for AHR, a ligand-activated transcription factor that plays important roles in immunity and cancer. AHR activation by indoles following IL4I1-mediated Trp degradation enhances tumor progression by promoting cancer cell motility and suppressing adaptive immunity. Also has an immunoregulatory function in some immune cell, probably by mediating Trp degradation and promoting downstream AHR activation: inhibits T-cell activation and proliferation, promotes the differentiation of naive CD4(+) T-cells into FOXP3(+) regulatory T-cells (Treg) and regulates the development and function of B-cells. Also regulates M2 macrophage polarization by inhibiting T-cell activation. Also has antibacterial properties by inhibiting growth of Gram negative and Gram positive bacteria through the production of NH4(+) and H2O2.
Gene References into Functions
  1. Data showed proteome changes in alveolar epithelial cells type II exposed to an infection with A. fumigatus with IL4I1 showing the most prominent increase in abundance both, in terms of relative abundance as well as activity. Also, in the infected lungs, there was levels of IL4I1 metabolic products. PMID: 28951444
Subcellular Location
Secreted. Cytoplasmic vesicle, secretory vesicle, acrosome.; [Isoform 1]: Lysosome.; [Isoform 2]: Lysosome.
Protein Families
Flavin monoamine oxidase family, FIG1 subfamily
Tissue Specificity
Primarily found in immune tissues.; [Isoform 1]: Primarily found in immune tissues, mostly in B-lymphocytes.; [Isoform 2]: Restricted to the testis, predominantly in Sertoli cells at the periphery of the ducts, and the brain, including Purkinje cells, hip
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