Mouse Lactadherin(MFGE8) ELISA kit

Code CSB-EL013752MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
milk fat globule-EGF factor 8 protein
Alternative Names
Mfge8Lactadherin ELISA Kit; MFGM ELISA Kit; Milk fat globule-EGF factor 8 ELISA Kit; MFG-E8 ELISA Kit; SED1 ELISA Kit; Sperm surface protein SP47 ELISA Kit; MP47 ELISA Kit
Uniprot No.
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
7.8 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MFGE8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 102
Range % 94-107
1:2 Average % 104
Range % 98-108
1:4 Average % 101
Range % 95-106
1:8 Average % 107
Range % 100-111
The recovery of mouse MFGE8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 86-97
EDTA plasma (n=4) 99 90-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.573 2.488 2.531 2.335
1000 1.845 1.898 1.872 1.676
500 1.122 1.156 1.139 0.943
250 0.649 0.631 0.640 0.444
125 0.467 0.453 0.460 0.264
62.5 0.344 0.337 0.341 0.145
31.25 0.289 0.282 0.286 0.090
0 0.193 0.199 0.196  
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Mouse MFGE8 ELISA Kit was designed for the quantitative measurement of Mouse MFGE8 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

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Target Background

(From Uniprot)
Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization.
Gene References into Functions
  1. study suggests that LPS represses MFG-E8 expression and disrupts enterocyte migration via a miR-99b dependent mechanism. PMID: 27538453
  2. Mesenchymal stem cell-derived MFG-E8 might accelerate diabetic wound healing by promoting angiogenesis, the clearance of apoptotic cells, and the infiltration of M2 macrophages, and by suppressing inflammatory cytokines in wound area. PMID: 28302404
  3. MFG-E8 localized to the basal lamina of the mammary gland during involution. Basal MFG-E8 localization promoted integrin-dependent suppression of beta-casein expression. PMID: 28884934
  4. MFG-E8 reduces neutrophil migration and neutrophil extracellular traps via downregulating surface CXCR2 expression in parallel with its role in the phagocytosis of apoptotic neutrophils. PMID: 27768123
  5. MFG-E8 administration was associated with decreased inflammasome activation in the kidney. Adoptive transfer of MFG-E8-stimulated macrophages reduced activation of inflammasome and tissue damage. The protective role of MFG-E8 in ureteral obstruction kidney injury is mediated through anti-inflammatory macrophage reprogramming which results in decreased inflammasome activation, preventing severe tissue damage. PMID: 27260955
  6. characterization of the mechanisms by which macrophage derived MFG-E8 regulates wound inflammation; wound macrophage-derived MFG-E8 was recognized as a critical driver of wound angiogenesis PMID: 27194784
  7. MFG-E8 acts at two levels, by increasing VEGF and ET-1 expression in mesenchymal stromal cells (MSC) and by enhancing M2 polarization of macrophages, to increase tumor angiogenesis. PMID: 27197197
  8. MFGE8 is an anti-fibrotic protein in mesenchymal stem cell secretomes that strongly inhibits TGFbeta signaling and reduces extracellular matrix deposition and liver fibrosis in mice. PMID: 27956229
  9. an efficient purification method for production of non-aggregated, full-length MFG-E8. PMID: 27102803
  10. MFG-E8 is a protective factor in the pathogenesis of rheumatoid arthritis and subsequent bone loss. PMID: 26391522
  11. Milk fat globule-epidermal growth factor-factor VIII downregulates interleukin-17 expression in sepsis by modulating STAT3 activation. PMID: 26376757
  12. MFG-E8 promotes tumor growth regardless of the presence or absence of colonic inflammation, whereas colon tumor development is initiated by MFG-E8 under inflammatory conditions. PMID: 25596854
  13. The MFG-E8 in breast milk may mask the phosphatidylserine exposed on the surface of mik fat globules(MFGs) with time after secretion and thereby suppress the membrane fusion among MFGs resulting in the enlargement of MFGs in the breast milk. PMID: 25661589
  14. data indicate that MFG-E8 controls steady-state and pathological bone turnover and may therefore represent a new target gene in the treatment of bone diseases PMID: 25868798
  15. MFG-8 is expressed in fetal hematopoietic stem cells populations, and MFG-E8 may have a role in embryonic hematopoiesis. PMID: 26206421
  16. increased TGFbeta1 expression in models of neurodegeneration might be involved in clearance of apoptotic cells via regulation of Milk fat globule-epidermal growth factor-factor 8 expression PMID: 25130376
  17. MFG-E8 may inhibit the formation of pressure ulcers induced by cutaneous I/R injury by regulating angiogenesis and inflammation PMID: 25493650
  18. results uncover a unique role of efferocytosis via MFG-E8 as a mechanism for macrophage polarization into tumor-promoting M2 cells PMID: 25006249
  19. MFG-E8 is a novel regulator that restrains RANKL-induced osteoclast differentiation and function with potential application of inhibiting experimental inflammatory bone loss. PMID: 24958900
  20. Mfge8 promotes obesity by mediating the uptake of dietary fats and serum fatty acids. PMID: 24441829
  21. These data demonstrate that the impairment of the phagocytic clearance of apoptotic cells through MFG-E8 can lead to the generation of natural antibodies, which may play a critical role in removing multiple damage-associated molecules. PMID: 23874637
  22. The balance between MFG-E8 and HMGB1 levels determines pathophysiological effects of chronic alcohol exposure on macrophage efferocytosis in vivo. PMID: 23552724
  23. beta-amyloid peptide induced neuronal loss is mediated by the bridging protein lactadherin/milk-fat globule epidermal growth factor-like factor 8 PMID: 23647050
  24. Increased MFG-E8 levels were associated with a significant increase in phagocytic activity resulting in apoptosis. PMID: 23405209
  25. our data argue that MFGE8 is not likely involved in the phagocytic clearance of neuronal debris associated with nigrostriatal pathway injury. PMID: 23194669
  26. GSPB2 treatment significantly decreased protein levels of MFG-E8, phospho-ERK1/2, phospho-Akt, and phospho-GSK-3beta in the kidneys of db/db mice. PMID: 23474305
  27. Data suggest that MFG-E8 (and phosphatidylserine PS) is present on surface of some milk fat globules (MFG) before weaning; after forced weaning, the surface of most MFG are MFG-E8-positive (and PS-negative); post-weaning MFG uptake is up-regulated. PMID: 23038672
  28. milk MFG-E8 was not equally present among fat globules and not necessarily intrinsic to the fat globules PMID: 23132565
  29. Liver and plasma MFG-E8 protein levels were significantly decreased after hepatic ischemia-reperfusion PMID: 22487387
  30. NMR solution structure of C2 domain of MFG-E8 and insights into its molecular recognition with phosphatidylserine PMID: 23262193
  31. Our findings define what we believe to a novel mechanism whereby MFG-E8 regulates the immunogenicity of DC by modulating the modes of recognition of dying cells. PMID: 22761839
  32. Mfge8 suppresses airway hyperresponsiveness in asthma by regulating smooth muscle contraction. PMID: 23269839
  33. MFG-E8 expression in the endometrial epithelium as well as in chorionic villi suggests its possible role in endometrial reorganization during the receptive phase and in events related to normal pregnancy in mammals. PMID: 22770563
  34. aged female mice deficient in both MFG-E8 and Tim4 developed autoantibodies in an age-dependent manner PMID: 22723547
  35. MFG-E8 significantly attenuates oligomeric amyloid beta-induced neuronal cell death in a primary neuron-microglia coculture system. PMID: 22742657
  36. MFG-E8 ameliorates neutrophil infiltration in acute inflammatory lung injury with extensive apoptotic cells. PMID: 22634615
  37. a key role of MFG-E8 release from apoptotic endothelial cells in macrophage reprogramming PMID: 22558449
  38. Mfge8(-/-) mice did not differ from controls in terms of fundus appearance, photoreceptor cell layers, choroidal architecture or laser-induced choroidal neovascularization. PMID: 22438901
  39. Phosphatidylserine-marked POS tips do not vary in mice lacking the diurnal phagocytic rhythm of the retinal epithelium due to loss of either alphavbeta5 integrin, or its extracellular ligand milk fat globule-EGF factor 8. PMID: 22566632
  40. We conclude that MFG-E8-dependent signaling stimulates cell proliferation and the acquisition of mesenchymal properties and contributes to mammary carcinoma development. PMID: 21841820
  41. This study showed that blocking MFG-E8-dependent phagocytosis preserves live neurons, implying that phagocytosis actively contributes to neuronal death during brain inflammation. PMID: 22357850
  42. MFG-E8 is an essential factor in attenuating inflammation and improving prognosis in several animal models of life threatening diseases. (Review) PMID: 21901532
  43. Data suggest that MGF-E8 is an essential protective factor for gut epithelial homeostasis, and exogenous administration of MFG-E8 may represent a novel therapeutic target in inflammatory bowel disease. PMID: 21308148
  44. studies strongly implicate MFG-E8 in pericyte/pericyte precursor function and indicate that MFG-E8-directed therapeutics may merit further development PMID: 21737783
  45. MFG-E8 controls phagocytic ingestion of cell fragments as well as their intracellular processing into MHC-antigen complexes. PMID: 21537078
  46. In a mouse model of carcinogen-induced bladder carcinoma, Mfge8s was up-regulated in mouse carcinoma. Mfge8-deficient mice developed less advanced tumors. Angiogenesis was similar in Mfge8-expressing or -deficient bladders. PMID: 20956946
  47. Mfge8 ablation induced accelerated prion disease PMID: 20837697
  48. analysis of SED1-null epididymis shows that loss of SED1 leads to an inability of the epididymal epithelium to properly regulate luminal fluid; SED1-null epididymal fluid is both hypo-osmotic and alkaline, relative to wildtype epididymal fluid PMID: 20422713
  49. MFG-E8 attenuates inflammation and ALI after gut I/R and may represent a novel therapeutic agent. PMID: 19892861
  50. Mfge8 directly bound collagen, and Mfge8-/- macrophages exhibited defective collagen uptake that could be rescued by recombinant Mfge8 containing at least one discoidin domain. PMID: 19884654

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Subcellular Location
Membrane; Peripheral membrane protein. Secreted. Cytoplasmic vesicle, secretory vesicle, acrosome membrane; Peripheral membrane protein.
Tissue Specificity
Mammary epithelial cell surfaces and spermatozoan. Isoform 2 is present in brain, heart, kidney and spleen and at low levels in lung, liver, small intestine and testis.
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