Mouse Leucine-rich repeat LGI family member 3(LGI3) ELISA kit

Instructions
Code CSB-EL012900MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name leucine-rich repeat LGI family, member 3
Alternative Names Lgi3Leucine-rich repeat LGI family member 3 ELISA Kit; Leubrin ELISA Kit; Leucine-rich glioma-inactivated protein 3 ELISA Kit
Abbreviation LGI3
Uniprot No. Q8K406
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LGI3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    Sample   Serum(n=4)  
1:1   Average %   95  
    Range %   90-100  
1:2   Average %   87  
    Range %   83-90  
1:4   Average %   102  
    Range %   98-108  
1:8   Average %   89  
    Range %   85-94  
Recovery
The recovery of mouse LGI3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type   Average % Recovery   Range  
Serum (n=5)   108   104-112  
EDTA plasma (n=4)   100   95-105  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.106 2.138 2.122 1.950  
1000 1.465 1.436 1.451 1.279  
500 0.895 0.918 0.907 0.735  
250 0.551 0.536 0.544 0.372  
125 0.329 0.331 0.330 0.158  
62.5 0.258 0.249 0.254 0.082  
31.25 0.201 0.210 0.206 0.034  
0 0.170 0.173 0.172    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function May participate in the regulation of neuronal exocytosis.
Gene References into Functions
  1. Taken together, these results support the critical involvement of LGI3 in the cytokine network by interplaying with multiple adipokines, cytokines and signaling proteins. PMID: 28534931
  2. findings show LGI3 and TNF-alpha upregulated each other in 3T3-L1 cells; study suggests LGI3 is a pro-inflammatory adipokine and forms a positive feedback loop with TNF-alpha; propose that LGI3 is a member of adipokine network that plays regulatory roles in obesity-associated adipose tissue inflammation PMID: 25648289
  3. results suggested that LGI3 may participate in adipose tissue homeostasis by negatively regulating adiponectin PMID: 23548727
  4. Data suggest that leucine-rich glioma inactivated 3 (LGI3) may be a candidate adipokine that is perturbed in obesity and suppresses adipogenesis through its receptor, ADAM23. PMID: 22405860
  5. LGI3 interacts with Flo1 in the brain, and LGI3/Flo1 mediates APP trafficking and exosome formation in neuronal cells: reveals the details of the intracellular transport system and even the secretion pathway in the brain. PMID: 20461023
  6. we propose that LGI3 is a neuritogenic factor whose signaling pathway involves Akt-mediated FAK activation. PMID: 20162351
  7. LGI3 gene widespread expression in the brain suggests that its transcripts might be involved in a common cellular process present in different neuronal types. PMID: 19833108
  8. The expression of mouse LGI3 (mLGI3) in adult and developing brain and analyzed the 5'-upstream transcriptional regulatory regions of mLGI3 gene. PMID: 16545924
  9. Data show that LGI3 localizes to the endocytic pathway and its accumulation is caused by endocytic perturbation. PMID: 18628660
  10. LGI3 may play a regulatory role in neuronal exocytosis via its interaction with syntaxin 1. PMID: 18760330

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Subcellular Location Secreted, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle, Cell junction, synapse, synaptosome
Database Links

KEGG: mmu:213469

STRING: 10090.ENSMUSP00000046705

UniGene: Mm.41708

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