Mouse Lysosomal Pro-X carboxypeptidase(PRCP) ELISA kit

Code CSB-EL018636MO
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
prolylcarboxypeptidase (angiotensinase C)
Alternative Names
Prcp ELISA Kit; Lysosomal Pro-X carboxypeptidase ELISA Kit; EC 3.4.16.2 ELISA Kit; Proline carboxypeptidase ELISA Kit; Prolylcarboxypeptidase ELISA Kit; PRCP ELISA Kit
Abbreviation
PRCP
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
37.5 pg/mL-2400 pg/mL
Sensitivity
9.37 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PRCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %85-99
1:2Average %98
Range %92-103
1:4Average %93
Range %89-98
1:8Average %91
Range %87-96
Recovery
The recovery of mouse PRCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-98
EDTA plasma (n=4)9493-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
24002.542 2.520 2.531 2.377
12002.017 1.955 1.986 1.832
6001.427 1.321 1.374 1.220
3000.722 0.781 0.752 0.598
1500.465 0.455 0.460 0.306
750.322 0.346 0.334 0.180
37.50.249 0.248 0.249 0.095
00.154 0.153 0.154
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse PRCP ELISA Kit was designed for the quantitative measurement of Mouse PRCP protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 37.5 pg/mL-2400 pg/mL and the sensitivity is 9.37 pg/mL.

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Target Background

Function
(From Uniprot)
Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.
Gene References into Functions
  1. Prolylcarboxypeptidase (PRCP) converts AngII to Ang (1-7) but only at an acidic pH. Global PRCP deficiency causes heart and kidney alterations and a moderate rise in BP. PRCP is abundant in the kidney collecting tubules, where the prevailing pH is low. In collecting tubules, PRCP deficiency could result in impaired AngII degradation. Increased AngII at this nephron site stimulates Na reabsorption and increases BP. PMID: 28160049
  2. activity and expression in plasma and lung tissue stays unaffected in lung ischemia-reperfusion injury PMID: 27437782
  3. This study demonistrated that PRCP gene is broadly expressed in the brain. PMID: 24161824
  4. PRCP regulates cell growth, angiogenesis, and the response to vascular injury PMID: 23744584
  5. PRCP is an important regulator of energy and glucose homeostasis since its deletion significantly improves metabolic parameters in mice exposed to both standard chow diet and high-fat diet PMID: 22454290
  6. analysis of non-benzimidazole and brain-penetrant prolylcarboxypeptidase inhibitors PMID: 22079761
  7. This study showed that PRCP mutant mice have a significant decrease in fat mass, although an increase in lean mass was also observed; reduced leptin levels and increased energy expenditure were also found. PMID: 22202165
  8. Murine prolylcarboxypeptidase depletion induces vascular dysfunction with hypertension and faster arterial thrombosis. PMID: 21297000
  9. PRCP is an important component of melanocortin signaling and weight maintenance via control of active alpha-MSH1-13 levels. PMID: 19620781

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Subcellular Location
Lysosome.
Protein Families
Peptidase S28 family
Database Links
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