Mouse Neurturin(NRTN) ELISA kit

Code CSB-EL016095MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
neurturin
Alternative Names
Nrtn ELISA Kit; Neurturin ELISA Kit
Abbreviation
NRTN
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.81 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse NRTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 81-89  
1:2 Average % 94  
Range % 90-98  
1:4 Average % 83  
Range % 80-86  
1:8 Average % 97  
Range % 94-101  
Recovery
The recovery of mouse NRTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 102 97-105  
EDTA plasma (n=4) 89 85-93  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.310 2.368 2.339 2.157  
1000 1.510 1.630 1.570 1.388  
500 0.885 0.875 0.880 0.698  
250 0.500 0.531 0.516 0.334  
125 0.368 0.378 0.373 0.191  
62.5 0.308 0.301 0.305 0.123  
31.25 0.240 0.250 0.245 0.063  
0 0.181 0.183 0.182    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse NRTN ELISA Kit was designed for the quantitative measurement of Mouse NRTN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

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Target Background

Function
(From Uniprot)
Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells.
Gene References into Functions
  1. These results indicate that NRTN contributes to an increase in the epidermal density of nonpeptidergic nerves in the imiquimod-induced psoriasis model, and this increase may be a factor in chronic itch. PMID: 28825602
  2. NRTN causes presynaptic and postsynaptic restoration of the nigrostriatal dopaminergic system after a 6-OHDA-induced chronic lesion. PMID: 29176874
  3. identified IFNg, Neurturin (Nrtn), and glial-derived neurotrophic factor (GDNF) as ligands with unexpected roles in promoting neurogenic differentiation of Neural Precursor Cells in vivo. PMID: 27545711
  4. These results demonstrate the potential roles of different functional groups of sensory neurons in the behavioral changes observed in mice overexpressing cutaneous neurturin and highlight the importance of neurturin in regulating cutaneous afferent response properties. PMID: 28031403
  5. Deletion of neurturin impairs cholinergic innervation of the heart and the baroreflex response in early postnatal development. PMID: 27162260
  6. Our findings support the hypothesis that NTN could modulate the allergic inflammation in different mouse asthma models. PMID: 25595789
  7. NTN inactivation enhances androgen sensitivity in reproductive and neuroendocrine tissues. PMID: 23678134
  8. the expression level of NRTN in the skin modulates gene expression in cutaneous sensory afferents and behavioral sensitivity to thermal, chemical, and mechanical stimuli. PMID: 23365243
  9. Findings provide evidence for a dampening role of NTN on allergic inflammation and AHR in a murine model of asthma. PMID: 21508262
  10. Transduction-function role for GFR alpha2/neurturin signalling in a specific class of sensory neurons. PMID: 12433948
  11. This study revealed the complexity of postnatal maturation of urogenital innervation, with each organ showing a distinct chronology of innervation and different requirement for neurturin. PMID: 18175352
  12. Neurturin-mediated Ret signaling is critical to normal retinal development and function. PMID: 18417692
  13. TGF-beta with neurturin and persephin are required for the induction of dopaminergic neurons, whereas GDNF is required for regulating and/or maintaining a differentiated neuronal phenotype PMID: 18420832
  14. Report structural and functional cardiac cholinergic deficits in adult neurturin knockout mice. PMID: 19176599

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Subcellular Location
Secreted.
Protein Families
TGF-beta family, GDNF subfamily
Tissue Specificity
Widespread distribution.
Database Links
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