Mouse Plastin-2(LCP1) ELISA kit

Code CSB-EL012824MO
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
lymphocyte cytosolic protein 1 (L-plastin)
Alternative Names
Lcp1 ELISA Kit; Pls2 ELISA Kit; Plastin-2 ELISA Kit; 65 kDa macrophage protein ELISA Kit; L-plastin ELISA Kit; Lymphocyte cytosolic protein 1 ELISA Kit; LCP-1 ELISA Kit; pp65 ELISA Kit
Abbreviation
LCP1
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
25 pg/mL-1600 pg/mL
Sensitivity
6.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-98  
1:2 Average % 99  
Range % 94-103  
1:4 Average % 84  
Range % 80-88  
1:8 Average % 90  
Range % 86-94  
Recovery
The recovery of mouse LCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 84-93  
EDTA plasma (n=4) 106 101-113  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1600 2.612 2.537 2.575 2.434  
800 2.142 2.056 2.099 1.958  
400 1.471 1.567 1.519 1.378  
200 0.874 0.905 0.890 0.749  
100 0.534 0.579 0.557 0.416  
50 0.290 0.279 0.285 0.144  
25 0.229 0.249 0.239 0.098  
0 0.144 0.137 0.141    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse LCP1 ELISA Kit was designed for the quantitative measurement of Mouse LCP1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

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Target Background

Function
(From Uniprot)
Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
Gene References into Functions
  1. the actin-bundling protein L-plastin (LPL) is required for the perinatal development of alveolar macrophages. PMID: 27758872
  2. Results identify L-plastin as a key effector of Mst1 and establish a novel mechanism linking a signaling intermediate to an actin-binding protein critical to T cell migration. PMID: 27465533
  3. L-plastin modulates both T- and B-cell function during the germinal center reaction and the production of T-cell-dependent antibody responses. PMID: 23589339
  4. L-plastin may participate in signaling that enables lymphocyte transmigration PMID: 21832165
  5. LPL-dependent actin bundling facilitates the formation of lamellipodia and normal immunological synapses and thereby enables T cell activation. PMID: 21076065
  6. Regulation of sealing ring formation by L-plastin and cortactin in osteoclasts. PMID: 20650888
  7. We thus identify L-plastin as a molecule critical for CCR7-mediated t-cell motility but dispensable for early CCR7 signaling. PMID: 20194718
  8. plastin plays a role in host defense distinguishes integrin signaling from cell adhesion and spreading. PMID: 12871642

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Subcellular Location
Cytoplasm, cytoskeleton. Cell projection. Cell junction. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side.
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