Product Details

Target Name

R-spondin 2 homolog (Xenopus laevis)

Alternative Names

Rspo2R-spondin-2 ELISA kit; Cysteine-rich and single thrombospondin domain-containing protein 2 ELISA kit; Cristin-2 ELISA kit; mCristin-2 ELISA kit; Roof plate-specific spondin-2 ELISA kit

Abbreviation

RSPO2

Uniprot No.

Q8BFU0

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.8 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Developmental Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse RSPO2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-102
1:2	Average %	91
	Range %	85-98
1:4	Average %	90
	Range %	88-93
1:8	Average %	100
	Range %	96-104

Recovery

The recovery of mouse RSPO2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-92
EDTA plasma (n=4)	106	103-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.522	2.633	2.578	2.472
1000	2.123	2.213	2.168	2.062
500	1.579	1.544	1.562	1.456
250	1.020	0.989	1.005	0.899
125	0.744	0.688	0.716	0.610
62.5	0.460	0.432	0.446	0.340
31.25	0.203	0.214	0.209	0.103
0	0.102	0.109	0.106

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse RSPO2 ELISA Kit was designed for the quantitative measurement of Mouse RSPO2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

Submit a Review here

Sample type: Biological fluids(Synovial fluid)

Sample species: Mouse

Sample dilution: No dilution

Review: We had success measuring R-spondin 2 in mouse serum, synovial fluid, and cell culture supernatant. The protocol was straightforward and after running a trial to test for optimal dilution, we proceeded with experimental samples.

By Alexander Knights

Read more reviews >>

Target Background

Function
(From Uniprot)

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors. During embryonic development, plays a crucial role in limb specification, amplifying the Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3, hence governing the number of limbs an embryo should form.

Gene References into Functions

Data report that Rspo2, is highly expressed in the spinal motor neuron (SMN) and binds to its receptor Lgr5, at the neuromuscular junction (NMJ). Rspo2 enhances the LRP4/MuSK signaling via Lgr5 in an agrin-independent manner and promotes AChR clustering. In addition, the loss of Rspo2 in mice compromises AChR clustering, the ultrastructure of the NMJ, and neuromuscular signal transduction. PMID: 27328992
Rspo2 activates Wnt/beta-catenin signaling to reduce Col2a1 and Sox9 and to facilitate differentiation of proliferating chondrocytes into hypertrophic chondrocytes in growth cartilage PMID: 27012200
High resolution crystal structure of an ectodomain variant of human LGR5 (hLGR5ecto) complexed with a signalling competent fragment of mouse Rspo2. PMID: 26123262
These findings suggest that R-Spondin2 may promote hepatic stellate cell activation by enhancing the canonical Wnt pathway. PMID: 24852883
R-spondin2 is a key molecular link between infection and intestinal homoeostasis. PMID: 23695692
R-spondin 2 homolog (Xenopus laevis) is identified as an essential regulator of Citrobacter rodentium infection susceptibility in mice PMID: 24005619
Oocyte-derived R-spondin2 is a paracrine factor essential for primary ovarian follicle development PMID: 23407710
Rspo2 is required to limit the number of outer hair cells to three rows and for optimal arrangement of peripheral nerve fibers in the developing cochlea. PMID: 23192966
Rspo2 mutation in combination with Rspo3 mutation causes severe limb truncation. PMID: 22610508
Recombinant R-spondin2 and Wnt3a up- and down-regulate novel target genes in C57MG mouse mammary epithelial cells. PMID: 22238613
Results indicate that Rspo2 and Wnt1 have mutually distinct effects on mammary epithelial cell growth and these effects are context-dependent. PMID: 21732367
Rspo2 is a mesenchyme-derived factor that plays critical roles in regulating branchial arch 1 patterning and morphogenesis through ectodermal-mesenchymal interaction and a novel genetic factor for cleft palate. PMID: 21237142
The findings suggest that Rspo2 expression in the apical ectodermal ridge (AER) is required for AER maintenance likely by regulating Wnt/beta-catenin signaling. PMID: 17904116
This study shows that Rspo2 is critical for maintenance of the AER and for growth and patterning in limb development. PMID: 18067586
Lrp6-mediated R-spondin 2 signaling through the canonical Wnt pathway is required for normal morphogenesis of the respiratory tract and limbs. PMID: 18256198
Wnt11 signals through beta-catenin, activating Rspo2 expression, which is then required for Wnt11-mediated osteoblast maturation. PMID: 19213727
These findings indicate that Rspo2 regulates midfacial, limb, and lung morphogenesis during development through the Wnt/beta-catenin signaling. PMID: 19233133
The Rspo2 mutants had markedly reduced potency relative to the wild-type protein,demonstrating for the first time specific amino acid residues in Rspos that are critical for beta-catenin signaling. PMID: 19385064

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Subcellular Location

Secreted.

Protein Families

R-spondin family

Database Links

KEGG: mmu:239405

STRING: 10090.ENSMUSP00000067325

UniGene: Mm.193274

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Mouse R-spondin-2(RSPO2) ELISA kit

Product Details

Citations

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Customer Reviews and Q&A

Target Background