Mouse Stanniocalcin-1(STC1) ELISA kit

Instructions
Code CSB-EL022821MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name stanniocalcin 1
Alternative Names Stc1 ELISA Kit; Stc ELISA Kit; Stanniocalcin-1 ELISA Kit; STC-1 ELISA Kit
Abbreviation STC1
Uniprot No. O55183
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse STC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 93    
Range % 87-97    
1:2 Average % 97    
Range % 95-102    
1:4 Average % 101    
Range % 95-105    
1:8 Average % 94    
Range % 91-98    
Recovery
The recovery of mouse STC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 92 87-98    
EDTA plasma (n=4) 99 95-104    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
4000 2.558 2.633 2.596 2.387    
2000 2.205 2.212 2.209 2.000    
1000 1.682 1.665 1.674 1.465    
500 0.990 0.995 0.993 0.784    
250 0.499 0.512 0.506 0.297    
125 0.390 0.421 0.406 0.197    
62.5 0.299 0.322 0.311 0.102    
0 0.205 0.212 0.209      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Stimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia.
Gene References into Functions
  1. Latanoprost-induced reduction of intraocular pressure is mediated through the downstream signaling molecule STC-1. When used by itself, STC-1 exhibits ocular hypotensive properties. PMID: 28538979
  2. STC-1 maybe improve anti-inflammation, anti-oxidant and anti-apoptosis activities by affecting reactive oxygen species -mediated pathways, especially the phospho-modifications of the respective proteins, resulting in the increase of Superoxide dismutase and reduce of capase-3, p53, IL-6 and IFN-gamma. PMID: 27420048
  3. STC1 blunts bleomycin-induced rise in thrombin protein and activity, diminishes thrombin-induced signaling through PAR1 to ERK, and inhibits bleomycin-induced pneumonitis. PMID: 26640170
  4. Stanniocalcin 1 is expressed in thyroid side population cells and thyroid cancer cells. PMID: 25647164
  5. Mesenchymal stem cells correct inappropriate epithelial-mesenchyme relation in pulmonary fibrosis using Stc1. PMID: 25373521
  6. Stanniocalcin-1 inhibits renal ischemia/reperfusion injury via an AMP-activated protein kinase-dependent pathway. PMID: 25012175
  7. STC1 is an endogenous stress protein that may counteract LPS-induced lung injury by inhibiting the inflammatory cascade and inducing antioxidant and antiapoptotic mechanisms. PMID: 24685991
  8. critical for kidney tubular epithelial survival under physiologic conditions PMID: 24700878
  9. Remarkably, X-linked genes are not overexpressed in female Stc1(-/-) mice, revealing the existence of a mechanism(s) that can compensate for a persistent XCI deficiency to regulate X-linked gene expression. PMID: 25136103
  10. Overexpression of stanniocalcin-1 inhibits reactive oxygen species and renal ischemia/reperfusion injury. PMID: 22695329
  11. STC1 was not crucial for the development of ischemic tolerance triggered by hypoxic preconditioning, or for preserving blood-brain barrier integrity but may be involved in functional recovery after stroke. PMID: 23159313
  12. the inner medullary STC-1 gene is differentially regulated in rat and mouse by arginine vasopressin and angiotensin II PMID: 21867741
  13. Vascular endothelial growth factor-D stimulates endothelial cell VEGF-A, stanniocalcin-1, and neuropilin-2 and has potent angiogenic effects. PMID: 21474827
  14. STC-1 could have a role in salt and water balance as dehydration necessitates water conservation as well as controlled natriuresis and kaliuresis. PMID: 20540985
  15. the biological repertoires of STCs in mammals will be considerably larger than in fish and may not be limited to mineral metabolism PMID: 11832417
  16. STC1 is expressed in a time- and cell-specific manner and may play an autocrine/paracrine role during osteoblast development and bone formation PMID: 11897801
  17. STC-1 can affect calcium homeostasis, bone and muscle mass and structure, and angiogenesis PMID: 12193584
  18. Data characterize the STC receptor and the functional targeting of ligand and receptor to mitochondria. PMID: 12223480
  19. STC1 may play a selective modulatory role in angiogenesis, possibly serving as a "stop signal" or stabilizing factor contributing to the maturation of newly formed blood vessels PMID: 14500721
  20. role for mammalian STC1 in modulating the immune/inflammatory response in ureteric obstruction PMID: 14570698
  21. kinetics of upregulated STC-1 expression during adipogenesis was investigated in 3T3-LI cells PMID: 15149855
  22. These findings have thus shown that the mammary glands are indeed targeted by STC, even in the virgin state. PMID: 16150955
  23. Stc1 function is not essential for growth or reproduction in the mouse PMID: 16287871
  24. systemically derived STC-1 has important effects on mammary gland development and the transfer of serum-based triglycerides into milk and also has a role in maternal behavior PMID: 16772321
  25. Hypoxic preconditioning leads to neuroprotection through IL-6-mediated upregulation of stanniocalcin-1 expression. PMID: 17272771
  26. IL-6-induced expression of STC-1 is one molecular mechanism behind the ischemic tolerance generated by HOPC in the heart PMID: 17573464
  27. stanniocalcin-1 is a potent anti-inflammatory and renal protective protein. PMID: 19246645
  28. Stc1 acts in a negative feedback loop in the prosurvival ERK1/2 signaling pathway during oxidative stress. PMID: 19347030
  29. Stanniocalcin-1 suppresses superoxide generation in macrophages through induction of mitochondrial UCP2. PMID: 19602668
  30. Transgenic overexpression of hSTC1 results in severe post-natal dwarfism (30-50%) indepedent of GH or IGF-I and a reduction female reproductive competence. PMID: 11861508

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Subcellular Location Secreted
Protein Families Stanniocalcin family
Tissue Specificity Expressed in many tissues.
Database Links

KEGG: mmu:20855

STRING: 10090.ENSMUSP00000014957

UniGene: Mm.20911

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