Mouse Transforming growth factor beta receptor type 3(TGFBR3) ELISA kit

Instructions
Code CSB-EL023453MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name transforming growth factor, beta receptor III
Alternative Names Tgfbr3 ELISA Kit; Transforming growth factor beta receptor type 3 ELISA Kit; TGF-beta receptor type 3 ELISA Kit; TGFR-3 ELISA Kit; Betaglycan ELISA Kit; Transforming growth factor beta receptor III ELISA Kit; TGF-beta receptor type III ELISA Kit
Abbreviation TGFBR3
Uniprot No. O88393
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGFBR3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 102
Range % 96-107
1:2 Average % 92
Range % 86-97
1:4 Average % 97
Range % 93-102
1:8 Average % 87
Range % 81-92
Recovery
The recovery of mouse TGFBR3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 98 94-102
EDTA plasma (n=4) 89 84-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
400 2.754 2.874 2.814 2.602
200 2.332 2.444 2.388 2.176
100 1.823 1.778 1.801 1.589
50 1.156 1.134 1.145 0.933
25 0.624 0.605 0.615 0.403
12.5 0.423 0.411 0.417 0.205
6.25 0.318 0.314 0.316 0.104
0 0.212 0.211 0.212  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Binds to TGF-beta. Could be involved in capturing and retaining TGF-beta for presentation to the signaling receptors (By similarity).
Gene References into Functions
  1. TGFbetaR3 promotes apoptosis of cardiomyocytes via a p38 pathway-associated mechanism, and loss of TGFbetaR3 reduces myocardial infarction injury. PMID: 28559372
  2. describe a detailed characterization of TbetaRIII expression in lymphocyte subpopulations demonstrating that this co-receptor is significantly expressed in T but not B lymphocytes and among them, preferentially expressed on naive and central memory T cells PMID: 29050936
  3. Study identified a novel major binding site for TGF-beta-like growth factors in TGFR-3. Findings potentially provide novel experimental insight into the general function of zona pellucida domain-containing proteins. PMID: 23826237
  4. a signaling pathway important in epicardial cell behavior downstream of TGFbetaR3 PMID: 27505173
  5. Retinoic acid enhanced TbetaRIII expression and that this increase contributed to LF-stimulated IgA production. PMID: 26277894
  6. These data reveal a critical, previously unrecognized role for TGFbetaR3 in vascular and osteoblast development during palatogenesis. PMID: 25382630
  7. These data demonstrate that TbetaRIII regulates BMP-mediated signaling and biological effects, primarily through the ligand sequestration effects of sTbetaRIII in normal and cancerous mammary epithelial cells. PMID: 25077702
  8. TGFBR3 mediated suppression of cancer progression includes effects on the tumor immune microenvironment. PMID: 23925295
  9. Loss of Tgfbr3 compromises the functions of TGFbeta2 in the differentiating fetal testis. PMID: 23303681
  10. A novel reciprocal loop between miR-21 and TGFbetaRIII in cardiac fibrosis caused by myocardial infarction in mice. PMID: 22960625
  11. These data suggest that Inhibins and TbetaRIII may function as a molecular pair regulating T cell development. PMID: 22952931
  12. the relative roles of TGFbetaR3-dependent and -independent signaling in the actions of BMP2 on epicardial cell behavior and demonstrate the critical role of TGFbetaR3 in mediating BMP2-stimulated invasion. PMID: 22237159
  13. used intact embryos and epicardial cells from E11.5 mouse embryos to reveal the mechanisms by which TGFbetaR3 signals and regulates epicardial cell behavior PMID: 21871877
  14. TGFBR3 can prevent hypoxia-induced injury in neonatal mice cardiac fibroblasts by negative regulation of TGF-beta signaling. PMID: 21792916
  15. Data present the 2.0-A resolution crystal structure of the betaglycan ZP-C region in combination with a downstream region known as the external hydrophobic patch. PMID: 21402931
  16. Tgfbr3 is associated with acute lung injury PMID: 21297076
  17. TbetaRIII enhanced both ALK3 & ALK6 signaling. TbetaRIII mediates differential ALK3 and ALK6 subcellular trafficking resulting in distinct signaling downstream of ALK3 and ALK6. PMID: 19726563
  18. required for normal seminiferous cord formation, normal fetal Leydig cell development, and the establishment of fetal testis endocrine function PMID: 19696014
  19. These results indicate that betaglycan expression is up-regulated during myogenesis and that MyoD and RA modulate its expression by a mechanism that is independent of myogenin. PMID: 12399463
  20. TbetaRIII is an important modulator of TGFbeta2 function in embryonic fibroblasts and reduced sensitivity to TGFbeta2 may underlie aspects of the TbetaRIII mutant phenotype PMID: 12773577
  21. TbetaRIII is not required for TGF-beta2 to signal when sufficient TGF-beta2, TbetaRI, and TbetaRII or TbetaRII-B are present PMID: 14996829
  22. TbetaRIII appeared to inhibit mammary tumor invasion by undergoing ectodomain shedding and producing soluble TbetaRIII. PMID: 17160136
  23. Tgfbr3 is required for coronary vessel development and embryonic viability. PMID: 17704211
  24. These data indicate that TbetaRIII acts as a tumor suppressor in nontumorigenic mammary epithelial cells at least in part by inhibiting NFkappaB-mediated repression of E-cadherin. PMID: 17823118
  25. Pronounced changes in the expression and localization of the TGF-beta receptors Tgfbr3 were noted as mouse lungs progressed through the canalicular, saccular, and alveolar stages of development. PMID: 18095342
  26. expressed at higher levels in the developing testis compared to ovary PMID: 18236212
  27. These results demonstrated that endocrine effects of inhibin as a negative feedback controller of follicle stimulating hormone production in gonadotropes are dependent on betaglycan expression. PMID: 19524135

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Subcellular Location Cell membrane, Single-pass type I membrane protein, Secreted, Secreted, extracellular space, extracellular matrix
Database Links

KEGG: mmu:21814

STRING: 10090.ENSMUSP00000031224

UniGene: Mm.200775

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