Mouse Translationally-controlled tumor protein(TPT1) ELISA kit

Code CSB-EL024134MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
tumor protein, translationally-controlled 1
Alternative Names
Tpt1 ELISA Kit; Trt ELISA Kit; Translationally-controlled tumor protein ELISA Kit; TCTP ELISA Kit; 21 kDa polypeptide ELISA Kit; p21 ELISA Kit; p23 ELISA Kit
Abbreviation
TPT1
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
9.37 pg/mL-600 pg/mL
Sensitivity
2.34 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %86
Range %80-91
1:2Average %93
Range %86-98
1:4Average %97
Range %92-102
1:8Average %94
Range %86-98
Recovery
The recovery of mouse TPT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9387-97
EDTA plasma (n=4)9088-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
6002.836 2.672 2.754 2.633
3002.099 2.184 2.142 2.021
1501.494 1.483 1.489 1.368
750.822 0.765 0.794 0.673
37.50.498 0.461 0.480 0.359
18.750.267 0.246 0.257 0.136
9.370.186 0.178 0.182 0.061
00.125 0.117 0.121  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse TPT1 ELISA Kit was designed for the quantitative measurement of Mouse TPT1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 9.37 pg/mL-600 pg/mL and the sensitivity is 2.34 pg/mL.

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 Q&A
Q:

Per customer request, can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.
d. Please send the current manual.
e. Please send your reference data for mouse plasma samples.

A:
Thanks for your inquiry!
CSB-EL024134MO
This kit is designed agaisnt full length protein. Pls refer to: according to: https://www.uniprot.org/uniprot/P63028.
Capture antibody is rat monoclonal antibody and detection antibody is goat polyclonal antibody.
Standard is 293 cell-derived recombinant protein.
Pls find attached manual.
We tested undiluted serum and plasma samples before and the test value is 40pg/ml.

Target Background

Function
(From Uniprot)
Involved in calcium binding and microtubule stabilization.
Gene References into Functions
  1. The biological effects of Na,K-ATPase and TCTP interaction are reviewed. PMID: 29867020
  2. that TPT1 knockdown altered the BECN1 interactome, a representative MTOR-independent pathway, to stimulate autophagosome formation PMID: 28409693
  3. The first crystal structure of murine HRF to 4.0A resolution, reveals a conserved fold. A model for the role of murine HRF in mast cell activation was created based on the human HRF structure. PMID: 29216544
  4. Fortilin directly interacts with the cytoplasmic domain of IRE1alpha, inhibits both kinase and endoribonuclease (RNase) activities of this stress sensor, and protects cells against apoptotic cell death at both cellular and whole animal levels. PMID: 28550308
  5. TCTP-mediated spindle dynamics play a key role in maintaining oocyte quality during postovulatory aging. Overexpression of TCTP is sufficient to prevent aging-associated abnormalities in mouse oocytes. PMID: 28476647
  6. Leads to the enhanced and previously un-described interaction of RACK1 and TCTP. PMID: 27816562
  7. These data suggest the presence of a novel oxidative-stress-handling pathway where the anti-p53 molecule fortilin augments the peroxidase PRX1 by protecting it against degradation and inactivation of the enzyme. PMID: 26726832
  8. results demonstrate that TCTP is a microtubule-associating protein required to regulate spindle microtubule dynamics during meiotic maturation in mouse oocytes. PMID: 26802898
  9. TCTP over-expression-induced RhoA activation indicates that silencing of cellular TCTP may offer therapeutic approaches for the regulation of hypertension by inhibiting RhoA/Rho kinase pathway and modulating vascular contractility. PMID: 24918292
  10. osteoclastogenic activity of translationally-controlled tumor protein (TCTP) with reciprocal repression of p21 PMID: 25263704
  11. Fortilin, induced in the proatherosclerotic microenvironment in macrophages, protects macrophages against Bax-induced apoptosis, allows them to propagate, and accelerates atherosclerosis. PMID: 24043250
  12. Overexpression of translationally controlled tumor protein increases the severity of atherosclerosis and hypertension in apolipoprotein E knock-out mice PMID: 22415346
  13. These findings suggest that TCTP might play an important role in the process of podocyte hypertrophy under diabetic conditions via the regulation of mTORC1 activity and the induction of cell-cycle arrest. PMID: 22311416
  14. The TCTP downregulates the Oct4 expression by binding the Sf1 site of Oct4 promoter in mouse pluripotent cells. PMID: 22281008
  15. TCTP may play a significant role in embryo implantation in mice PMID: 21865235
  16. knockdown of Tctp resulted in increased amounts of P53 accompanied by a 50% reduction in the efficiency of tumor mammosphere formation PMID: 22157679
  17. Identified a subset of IgE and IgG antibodies as TCTP(HRF)-interacting molecules in vitro. HRF was able to dimerize and bind to Igs via interactions of its N-terminal and internal regions with the Fab region of Igs. PMID: 22133880
  18. Data indicate that Hprt, Rpl13a and Tpt1 are a set of stably expressed reference genes for accurate gene expression normalization in myocardial infarction studies in mice. PMID: 21858224
  19. The Tpt1-Npm1 complex as a novel marker for highly proliferating cells, and offer further insight into the network that controls cellular fate. PMID: 20505363
  20. Results imply that TCTP levels influence the sensitivity of beta cells to apoptosis PMID: 21063673
  21. TCTP levels influence sensitivity to apoptosis PMID: 19901967
  22. Plk phosphorylates TCTP on 2 Ser residues in vitro & cofractionates with the majority of kinase activity toward TCTP in mitotic cell lysates. Phosphorylation decreases its microtubule-stabilizing activity & promotes increased MT dynamics after metaphase. PMID: 12167714
  23. seven putative Tpt1 genes with different chromosomal localizations were identified; analysis of sequence, genomic organization, and in vitro promoter activity. PMID: 12782126
  24. TCTP stabilizes Mcl-1 through interfering with Mcl-1's degradation by the ubiquitin-dependent proteasome degradation pathway and modulates its antiapoptotic activity. PMID: 15798198
  25. an N-terminal region of a cytosolic protein, TCTP, is required for its binding to Bcl-xL and for its antiapoptotic activity PMID: 15870695
  26. These results suggest that despite that TCTP is widely expressed in many tissues or cell types, it appears to regulate cell proliferation and survival in a tissue- or cell type-specific manner. PMID: 17475776
  27. Fortilin is an intracellular Ca2+ scavenger, protecting cells against Ca2+-dependent apoptosis by binding and sequestering divalent calcium ions (Ca2+) from the downstream Ca2+-dependent apoptotic pathways. PMID: 17705784
  28. TCTP antisense expression in CD25(high) regulatory T cells aggravates cuff-injured vascular inflammation PMID: 18789801
  29. In early phase of development, fortilin functions as an inhibitor of the bone morphogenetic protein pathway. PMID: 19364479
  30. Study demonstrated that TCTP acts as a cataractogenic factor through the repression of Na,K-ATPase activity and calcium mobilization in lens epithelial cells. PMID: 19479337
  31. Proton pump inhibitors exert anti-allergic effects by reducing TCTP secretion PMID: 19484128
  32. TCTP plays a very modest role in thymocyte development, but is critical for peripheral T cell maintenance and T cell receptor (TCR)-mediated cell proliferation. PMID: 19605695

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Subcellular Location
Cytoplasm.
Protein Families
TCTP family
Database Links
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