TPT1

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse liver tissue
All lanes: TPT1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 20, 16 kDa
Observed band size: 25 kDa

IHC image of CSB-RA214107A0HU diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of SY5Y Cells with CSB-RA214107A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunoprecipitating TCTP in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA214107A0HU in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA214107A0HU(2µg)+ Hela whole cell lysate(500µg)
Lane 3: Hela whole cell lysate (10µg)