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Mouse Tryptase beta-2(TPSB2) ELISA kit

Mouse Tryptase beta-2(TPSB2) ELISA kit

Citations (3) Protocol MSDS

Code	CSB-EL024128MO
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (3)
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Customer Reviews and Q&A
Target Background

Product Details

Target Name

tryptase beta 2

Alternative Names

Tpsb2 ELISA Kit; Mcpt6 ELISA Kit; Tryptase beta-2 ELISA Kit; Tryptase-2 ELISA Kit; EC 3.4.21.59 ELISA Kit; Mast cell protease 6 ELISA Kit; mMCP-6 ELISA Kit

Abbreviation

TPSB2

Uniprot No.

P21845

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

78 pg/mL-5000 pg/mL

Sensitivity

19.5 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPSB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Recovery

The recovery of mouse TPSB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Mouse TPSB2 ELISA Kit was designed for the quantitative measurement of Mouse TPSB2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

Citations

Neuronal substance P-driven MRGPRX2-dependent mast cell degranulation products differentially promote vascular permeability 長嶺誠和，ナガミネマサカズ,Thesis & Dissertation,2024
Neuronal substance P-driven MRGPRX2-dependent mast cell degranulation products differentially promote vascular permeability M Nagamine, A Kaitani, K Izawa, T Ando,Frontiers in Immunology,2024
Activation of Mast-Cell-Expressed Mas-Related G-Protein-Coupled Receptors Drives Non-histaminergic Itch Meixiong J,Immunity,2019

Customer Reviews and Q&A

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Target Background

Function
(From Uniprot)

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Plays a role in innate immunity.

Subcellular Location

Secreted.

Protein Families

Peptidase S1 family, Tryptase subfamily

Tissue Specificity

During embryogenesis, detected primarily in skin.

Database Links

STRING: 10090.ENSMUSP00000063499

UniGene: Mm.7409

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ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

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Mouse Tryptase beta-2(TPSB2) ELISA kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background