Mouse Tryptase beta-2(TPSB2) ELISA kit

Code CSB-EL024128MO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P21845
Lead Time 7-14 working days
Abbreviation TPSB2
Target Name tryptase beta 2
Alias TPS2, tryptaseB, tryptaseC, OTTHUMP00000208944|mast cell tryptase beta II|mast cell tryptase beta III|tryptase II|tryptase III|tryptase beta 2|tryptase beta II|tryptase beta III
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3 UTR and contain tandem repeat sequences at the 5 flank and 3 UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, beta II and beta III. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha-tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess. 

 
Linearity To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPSB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Recovery The recovery of mouse TPSB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Typical Data These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 





References
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Function Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Plays a role in innate immunity.
Subcellular Location Secreted
Protein Families Peptidase S1 family, Tryptase subfamily
Tissue Specificity During embryogenesis, detected primarily in skin.
Database Links

STRING: 10090.ENSMUSP00000063499

UniGene: Mm.7409

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