Product Details

Target Name

alpha-1-microglobulin/bikunin precursor (AMBP)

Alternative Names

Ambp ELISA Kit; ItilProtein AMBP [Cleaved into: Alpha-1-microglobulin; Inter-alpha-trypsin inhibitor light chain ELISA Kit; ITI-LC ELISA Kit; Bikunin ELISA Kit; HI-30); Trypstatin] ELISA Kit

Abbreviation

AMBP

Uniprot No.

Q07456

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

1.563 ng/mL-100 ng/mL

Sensitivity

0.649 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Competitive

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AMBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	104
	Range %	101-107
1:200	Average %	96
	Range %	93-99
1:400	Average %	94
	Range %	90-97
1:800	Average %	91
	Range %	84-95

Recovery

The recovery of mouse AMBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-93
EDTA plasma (n=4)	97	92-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
100	0.140	0.145	0.143
50	0.357	0.366	0.362
25	0.569	0.578	0.574
12.5	0.865	0.898	0.882
6.25	1.544	1.532	1.538
3.125	2.083	2.098	2.091
1.563	2.469	2.466	2.468
0	2.844	2.878	2.861

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Data

Function	Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization (By similarity).
Gene References into Functions	Loss of bikunin expression is associated with Embryos failing to Initiate Midgut Rotation. PMID: 30174180 In this review, the use of genetically targeted mice selectively lacking UTI provides evidence of a therapeutic option for experimental endotoxin-related inflammatory disorders such as acute lung injury and acute liver injury. PMID: 20367192 expression of the AMBP gene transcript and its two protein products, alpha(1)-microglobulin and bikunin, in embryogenesis PMID: 12204273 UTI inhibited LPS-induced production of TNF-alpha by inhibiting activation of the extracellular signal-regulated protein kinases 1/2-Egr-1 pathway in monocytes, which might at least partly contribute to reduction of hypotension PMID: 15539418 UTI protects against systemic inflammatory response and subsequent organ injury induced by bacterial endotoxin. PMID: 15576631 results indicate that endogenous UTI is protective against acute lung injury induced by bacterial endotoxin, at least partly, via the antioxidative properties PMID: 16273282 increased sensitivity of Bik-/- mice to lung metastasis in vivo is due to a lack of circulating proteins of the inter-alpha inhibitor family, especially bikunin PMID: 16331631 UTI reduces NF-kappaB activation and downregulates the expression of its related mediators, followed by the inhibition of neutrophil aggregation and infiltration in hepatic ischemia reperfusion injury PMID: 19208516 Report impaired liver regeneration with humoral and genetic disturbances in urinary trypsin inhibitor-deficient mice. PMID: 19302180 UTI protects against severe liver injury and subsequent coagulatory disturbance which was mediated, at least partly, through the suppression of TNF-alpha production along with its antiprotease activity. PMID: 19398962 Show More Hide All
Subcellular Location	Secreted
Protein Families	Calycin superfamily, Lipocalin family
Tissue Specificity	Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
Database Links	KEGG: mmu:11699 STRING: 10090.ENSMUSP00000030041 UniGene: Mm.2197

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Check Code：

Code	CSB-E09776m
Size	96T,5×96T,10×96T
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Mouse alpha-1-microglobulin/bikunin precursor (AMBP) ELISA kit

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