Mouse alpha-1-microglobulin/bikunin precursor (AMBP) ELISA kit

Instructions
Code CSB-E09776m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name alpha-1-microglobulin/bikunin precursor (AMBP)
Alternative Names Ambp ELISA Kit; ItilProtein AMBP [Cleaved into: Alpha-1-microglobulin; Inter-alpha-trypsin inhibitor light chain ELISA Kit; ITI-LC ELISA Kit; Bikunin ELISA Kit; HI-30); Trypstatin] ELISA Kit
Abbreviation AMBP
Uniprot No. Q07456
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.563 ng/mL-100 ng/mL
Sensitivity 0.649 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse AMBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 104
Range % 101-107
1:200 Average % 96
Range % 93-99
1:400 Average % 94
Range % 90-97
1:800 Average % 91
Range % 84-95
Recovery
The recovery of mouse AMBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 87-93
EDTA plasma (n=4) 97 92-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
100 0.140 0.145 0.143
50 0.357 0.366 0.362
25 0.569 0.578 0.574
12.5 0.865 0.898 0.882
6.25 1.544 1.532 1.538
3.125 2.083 2.098 2.091
1.563 2.469 2.466 2.468
0 2.844 2.878 2.861
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization (By similarity).
Gene References into Functions
  1. Loss of bikunin expression is associated with Embryos failing to Initiate Midgut Rotation. PMID: 30174180
  2. In this review, the use of genetically targeted mice selectively lacking UTI provides evidence of a therapeutic option for experimental endotoxin-related inflammatory disorders such as acute lung injury and acute liver injury. PMID: 20367192
  3. expression of the AMBP gene transcript and its two protein products, alpha(1)-microglobulin and bikunin, in embryogenesis PMID: 12204273
  4. UTI inhibited LPS-induced production of TNF-alpha by inhibiting activation of the extracellular signal-regulated protein kinases 1/2-Egr-1 pathway in monocytes, which might at least partly contribute to reduction of hypotension PMID: 15539418
  5. UTI protects against systemic inflammatory response and subsequent organ injury induced by bacterial endotoxin. PMID: 15576631
  6. results indicate that endogenous UTI is protective against acute lung injury induced by bacterial endotoxin, at least partly, via the antioxidative properties PMID: 16273282
  7. increased sensitivity of Bik-/- mice to lung metastasis in vivo is due to a lack of circulating proteins of the inter-alpha inhibitor family, especially bikunin PMID: 16331631
  8. UTI reduces NF-kappaB activation and downregulates the expression of its related mediators, followed by the inhibition of neutrophil aggregation and infiltration in hepatic ischemia reperfusion injury PMID: 19208516
  9. Report impaired liver regeneration with humoral and genetic disturbances in urinary trypsin inhibitor-deficient mice. PMID: 19302180
  10. UTI protects against severe liver injury and subsequent coagulatory disturbance which was mediated, at least partly, through the suppression of TNF-alpha production along with its antiprotease activity. PMID: 19398962

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Subcellular Location Secreted
Protein Families Calycin superfamily, Lipocalin family
Tissue Specificity Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
Database Links

KEGG: mmu:11699

STRING: 10090.ENSMUSP00000030041

UniGene: Mm.2197

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