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Mouse alpha-1-microglobulin/bikunin precursor (AMBP) ELISA kit

Mouse alpha-1-microglobulin/bikunin precursor (AMBP) ELISA kit

Specification
Target

Code

CSB-E09776m

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

Q07456

Lead Time

3-5 working days

Abbreviation

AMBP

Protein Biological Process 1

Tumor marker

Target Name

alpha-1-microglobulin/bikunin precursor (AMBP)

Alias

A1M, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI, alpha-1-microglobulin/bikunin|bikunin|complex-forming glycoprotein heterogeneous in charge|growth-inhibiting protein 19|inter-alpha-trypsin inhibi

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

1.563 ng/mL-100 ng/mL

Sensitivity

0.649 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Competitive

Target Details

This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AMBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	104
	Range %	101-107
1:200	Average %	96
	Range %	93-99
1:400	Average %	94
	Range %	90-97
1:800	Average %	91
	Range %	84-95

Recovery

The recovery of mouse AMBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-93
EDTA plasma (n=4)	97	92-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
100	0.140	0.145	0.143
50	0.357	0.366	0.362
25	0.569	0.578	0.574
12.5	0.865	0.898	0.882
6.25	1.544	1.532	1.538
3.125	2.083	2.098	2.091
1.563	2.469	2.466	2.468
0	2.844	2.878	2.861

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Function	Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization (By similarity).
Subcellular Location	Secreted
Protein Families	Calycin superfamily, Lipocalin family
Tissue Specificity	Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
Database Links	KEGG: mmu:11699 STRING: 10090.ENSMUSP00000030041 UniGene: Mm.2197

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Mouse alpha-1-microglobulin/bikunin precursor (AMBP) ELISA kit

Specification

Target

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