Mouse anti-double stranded DNA antibody(IgM)ELISA Kit

Code CSB-E12911m
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse dsDNA Ab (IgM) ELISA Kit was designed for the quantitative measurement of Mouse dsDNA Ab (IgM) protein in serum, plasma. It is a Capture ELISA ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

Target Name anti-double stranded DNA antibody(IgM)
Abbreviation dsDNA Ab (IgM)
Species Mus musculus (Mouse)
Sample Types serum, plasma
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Capture ELISA
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-double stranded DNA antibody (IgM) (dsDNA Ab (IgM)) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %95
Range %85-105
1:2Average %89
Range %85-98
1:4Average %93
Range %85-97
1:8Average %95
Range %89-110
Recovery
The recovery of mouse anti-double stranded DNA antibody (IgM) (dsDNA Ab (IgM)) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9483-101
EDTA plasma (n=4)9787-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1002.501 2.531 2.516 2.377
502.178 2.203 2.191 2.052
251.803 1.822 1.813 1.674
12.51.254 1.268 1.261 1.122
6.250.731 0.745 0.738 0.599
3.120.405 0.397 0.401 0.262
1.560.255 0.249 0.252 0.113
00.138 0.140 0.139
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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