Mouse anti-nuclear Antibody (IgG) ELISA Kit

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Name: Mouse anti-nuclear Antibody (IgG) ELISA Kit
Brand: CUSABIO
SKU: CSB-E12912m

Product Details

Description

This Mouse ANA Ab (IgG) ELISA Kit was designed for the quantitative measurement of Mouse ANA Ab (IgG) protein in serum, plasma. It is a Capture ELISA ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .

Target Name

anti-nuclear Antibody ( IgG)

Abbreviation

ANA Ab (IgG)

Species

Mus musculus (Mouse)

Sample Types

serum, plasma

Detection Range

7.8 pg/mL-500 pg/mL

Sensitivity

1.95 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Capture ELISA

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-nuclear antibody(IgG) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	84
	Range %	80-92
1:200	Average %	97
	Range %	91-105
1:400	Average %	85
	Range %	82-89
1:800	Average %	93
	Range %	86-98

Recovery

The recovery of mouse anti-nuclear antibody(IgG) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	92	87-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.751	2.752	2.752	2.559
250	2.327	2.377	2.352	2.159
125	1.700	1.675	1.688	1.495
62.5	0.993	0.968	0.981	0.788
31.2	0.637	0.625	0.631	0.438
15.6	0.401	0.416	0.409	0.216
7.8	0.299	0.289	0.294	0.101
0	0.188	0.197	0.193

Materials provided

A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse IgG antibody.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled antigen(100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-conjugateDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

Antibodies for which exactly anti-nuclear proteins are analyzed in this kit?

Thanks for your inquiry.
The kit CSB-E12912m is quantitative determination the general quantity of anti-nuclear antibody (IgG).Pls let me know if you have any further questions. Thank you.

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Check Code：

Code	CSB-E12912m
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.