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Mouse anti-thyroid-globulin antibody,TGAB ELISA Kit

Mouse anti-thyroid-globulin antibody,TGAB ELISA Kit

Citations (2) Protocol

Code	CSB-E09543m
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
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Product Details
Citations (2)
Customer Reviews and Q&A

Product Details

Target Name

anti-thyroid-globulin antibody,TGAB

Abbreviation

TG Ab

Species

Mus musculus (Mouse)

Sample Types

serum, plasma

Detection Range

1.25 U/mL-20 U/mL

Sensitivity

1.25 U/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Competitive

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGAB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-101
1:2	Average %	89
	Range %	85-98
1:4	Average %	92
	Range %	84-100
1:8	Average %	95
	Range %	90-100

Recovery

The recovery of mouse TGAB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-103
EDTA plasma (n=4)	95	90-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

U/ml	OD1	OD2	Average
20	0.146	0.151	0.149
10	0.278	0.281	0.280
5	0.457	0.463	0.460
2.5	0.854	0.870	0.862
1.25	1.865	1.828	1.847

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse TG Ab ELISA Kit was designed for the quantitative measurement of Mouse TG Ab protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 1.25 U/mL-20 U/mL and the sensitivity is 1.25 U/mL.

Citations

Prunella vulgaris can improve the pregnancy outcomes of experimental autoimmune thyroiditis rats by inhibiting Th1/Th17 immune responses Q Zhu,Journal of Reproductive Immunology,2023
Human amnion-derived mesenchymal stem cells improve subclinical hypothyroidism by immunocompetence mediating apoptosis inhibition on thyroid cells in aged mice C Li,Cell and tissue research,2023

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

Submit a Review here

Sample type: Serum

Sample species: Mouse

Sample dilution: 1: 10

Review: I used CSB-E09543m to detect autoimmune thyroiditis rats, and the dilution ratio was 1: 10. The experimental results were also accurate and in line with the trend. We expect to produce the TGAB box for rats, and there is a great demand in our laboratory.

By Anonymous

Read more reviews >>

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