Mouse bone morphogenetic protein 9,BMP-9 ELISA Kit

Instructions
Code CSB-E14282m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name growth differentiation factor 2
Alternative Names Gdf2 ELISA Kit; Bmp9Growth/differentiation factor 2 ELISA Kit; GDF-2 ELISA Kit; Bone morphogenetic protein 9 ELISA Kit; BMP-9 ELISA Kit
Abbreviation GDF2
Uniprot No. Q9WV56
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.312 pg/mL-20 pg/mL
Sensitivity 0.078 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %100
Range %91-104
1:2Average %104
Range %97-108
1:4Average %98
Range %89-102
1:8Average %94
Range %89-99
Recovery
The recovery of mouse BMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9989-103
EDTA plasma (n=4)9485-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
202.266 2.231 2.249 2.081
101.389 1.296 1.343 1.175
50.850 0.834 0.842 0.674
2.50.582 0.531 0.557 0.389
1.250.422 0.441 0.432 0.264
0.6250.316 0.303 0.310 0.142
0.3120.249 0.233 0.241 0.073
00.170 0.166 0.168
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Potent circulating inhibitor of angiogenesis (By similarity). Signals through the type I activin receptor ACVRL1 but not other Alks
Gene References into Functions
  1. Low BMP9 expression is associated with breast cancer. PMID: 30015950
  2. These results suggest that RUNX1 may be an essential modulator in BMP9- induced osteogenic differentiation of mesenchymal stem cells. PMID: 28644396
  3. results provide a better understanding into how BMP-9 induces osteoblast differentiation and its synergy with IGF-2 at the signaling level. PMID: 27477105
  4. Our findings provide a clearer understanding of the cellular pathways utilized by BMP-9 for chondrogenesis that may help improve current therapies for regenerative cartilage repair. PMID: 27056281
  5. Constitutive expression of low levels of BMP-9 stabilises hepatocyte function in the healthy liver. High levels of BMP-9 cause enhanced damage upon acute or chronic injury. PMID: 28336518
  6. Notch signaling may play an important role in BMP9-induced osteogenesis and angiogenesis. It's conceivable that simultaneous activation of the BMP9 and Notch pathways should efficiently couple osteogenesis and angiogenesis of MSCs for successful bone tissue engineering. PMID: 28384643
  7. he results of the present study demonstrated that BMP9 promoted the osteoclast differentiation of osteoclast precursors via binding to the ALK1 receptor on the cell surface, and inhibiting the ERK1/2 signaling pathways in the cell PMID: 27748860
  8. that Dkk1 negatively regulates BMP9-induced osteogenic differentiation. PMID: 26674341
  9. Data show athat beta-catenin can be activated by bone morphogenetic protein 9 (BMP9) and the activation of beta-catenin plays an important role in the differentiation of C3H10T1/2 cells into cardiomyocyte-like cells induced by BMP9. PMID: 27371840
  10. miR23b inhibits BMP9induced C2C12 myoblast osteogenesis via targeting of the Runx2 gene, acting as a suppressor. PMID: 26820568
  11. We have established a producer line that stably expresses a high level of active BMP9 protein. Such producer line should be a valuable resource for generating biologically active BMP9 protein for studying BMP9 signaling PMID: 26816490
  12. Data show that microRNA miR-21 was significantly upregulated by bone morphogenetic protein 9 (BMP9) during the osteogenesis the multilineage cells (MMCs) by suppressing Smad7 protein. PMID: 26460584
  13. Hh signaling is involved and plays a regulatory role in the osteogenic differentiation of MSCs induced by BMP9. PMID: 25872645
  14. data indicate that BMP9 and BMP13 (BMP9 might be more effective) promoted the differentiation of C3H10T1/2 cells into cardiomyocyte-like cells PMID: 24380493
  15. BMP9/ALK1 augmented vasculogenesis and angiogenesis, and thereby enhanced neovascularization. Thus, we suggest that BMP9/ALK1 may improve the efficacy of EPC-based therapies for treating ischemic diseases. PMID: 26229139
  16. BMP-9 induces vascular smooth muscle cell osteogenic differentiation and calcification via ALK1, Smad and ALP dependent mechanisms. PMID: 25297851
  17. These findings suggest that PTEN plays an important role in regulating BMP9 induced osteogenic differentiation in MPCs, which may be mediated by PTEN/PI3K/Akt signaling to modulate the expression of COX-2. PMID: 25176064
  18. BMP9 may be explored as a novel and efficacious factor for odontogenic regeneration. PMID: 24517722
  19. IGF2 increased the protein levels of hippocampal BMP9, NGF, BDNF, NT3 and IGF1 and of doublecortin, a marker of neurogenesis. PMID: 24732467
  20. Data indicate that extracellular signal-regulated kinase 5 (ERK5) and c-Jun N-terminal kinase (JNK) are important in the differentiation of C3H10T1/2 cells into cardiomyocyte-like cells induced by bone morphogenetic protein 9 (BMP9) transfection. PMID: 25108436
  21. results strongly suggest that Creld2 may be directly regulated by BMP9 and ER stress response may play an important role in regulating osteogenic differentiation PMID: 24019898
  22. FGF2 inhibited BMP9-induced osteogenic differentiation by blocking BMP9-induced Smads signaling and subsequently reducing Smads dependent up-regulation of ALK1 and ALK2 in mesenchymal stem cells. PMID: 23680673
  23. BMP9 ameliorates amyloidosis and the cholinergic defect in a mouse model of Alzheimer's disease. PMID: 24218590
  24. Bmp9 mediates the inhibitory effects on lymphatic vessel formation of ALK-1 signaling. PMID: 24133138
  25. BMP-9 maintains epithelial polarity via intracellular signaling from basolaterally localized BMP receptors. PMID: 23675417
  26. BMP9 is an important extracellular regulator in the maturation of the lymphatic vascular network affecting valve development and lymphatic vessel function. PMID: 23741013
  27. BMP-9 induced osteogenic differentiation of dental follicle stem cells depended on MAPK signaling pathway. PMID: 23155360
  28. in an in vitro model of HHT2, loss of Alk1 blocks BMP9 signaling, resulting in reduced EphrinB2 expression, enhanced VEGFR2 expression, and misregulated EC sprouting and anastomosis PMID: 22622516
  29. Growth hormone synergizes with BMP9 in osteogenic differentiation by activating the JAK/STAT/IGF1 pathway in murine multilineage cells PMID: 22467218
  30. platelets regulate blood/lymphatic vessel separation by inhibiting the proliferation, migration, and tube formation of LECs, mainly because of the release of BMP-9 upon activation by CLEC-2/podoplanin in PMID: 22556408
  31. During mouse development circulating levels of BMP9 peaked during the first 3 weeks after birth and then decreased to 2 ng/mL in adulthood. PMID: 21710321
  32. Results demonstrated that BMPRII and ActRII are the functional type II TGF-beta receptors in BMP-9-induced osteogenic differentiation of C3H10T1/2 cells. PMID: 20801928
  33. BMP-9 crosstalks with IGF-2 through PI3K/AKT signaling pathway during osteogenic differentiation of mesenchymal stem cells. PMID: 20499340
  34. Chromatin immunoprecipitation (ChIP) analysis indicated that BMP-9 induced recruitment of both Runx2 and beta-catenin to the osteocalcin promoter PMID: 19175684
  35. Therefore, pBMP-9 might be a promising replacement for costly BMP in tissue engineering applications that require a well-defined serum-free medium. PMID: 19388833
  36. ALK-1, an orphan receptor in the TGF-beta family, is a potential receptor for BMP-9 PMID: 15851468
  37. BMP9 induces the transcriptome of basal forebrain cholinergic neurons PMID: 15870197
  38. Hey1 and Runx2 were shown to act synergistically in BMP9-induced osteogenic differentiation, and Runx2 expression significantly decreased in the absence of Hey1, suggesting that Runx2 may function downstream of Hey1 PMID: 18986983

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Subcellular Location Secreted
Protein Families TGF-beta family
Database Links

KEGG: mmu:12165

STRING: 10090.ENSMUSP00000098286

UniGene: Mm.422844

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