Mouse choline acetyltransterase(ChAT)ELISA Kit

Code CSB-E17524m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
choline acetyltransterase(ChAT
Alternative Names
ChatCholine O-acetyltransferase ELISA kit; CHOACTase ELISA kit; ChAT ELISA kit; Choline acetylase ELISA kit; EC ELISA kit
Uniprot No.
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell culture supernates
Detection Range
15.6 pg/mL-1000 pg/mL
3.9 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ChAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:100 Average % 92    
Range % 88-96    
1:200 Average % 87    
Range % 82-91    
1:400 Average % 103    
Range % 99-108    
1:800 Average % 95    
Range % 90-100    
The recovery of mouse ChAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
Sample Type Average % Recovery Range    
Serum (n=5) 96 91-100    
EDTA plasma (n=4) 92 88-96    
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
pg/ml OD1 OD2 Average Corrected    
1000 2.042 2.136 2.089 1.933    
500 1.407 1.481 1.444 1.288    
250 0.893 0.947 0.920 0.764    
125 0.610 0.658 0.634 0.478    
62.5 0.475 0.463 0.469 0.313    
31.2 0.321 0.305 0.313 0.157    
15.6 0.254 0.249 0.252 0.096    
0 0.159 0.152 0.156      
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days

This Mouse CHAT ELISA Kit was designed for the quantitative measurement of Mouse CHAT protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Target Background

(From Uniprot)
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Protein Families
Carnitine/choline acetyltransferase family
Database Links
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