Mouse circulating immune complex(CIC)ELISA Kit

Code CSB-E16553m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
circulating immune complex(CIC)
Alternative Names
N/A
Abbreviation
CIC
Species
Mus musculus (Mouse)
Sample Types
serum, plasma
Detection Range
15.6 ng/mL-1000 ng/mL
Sensitivity
3.9 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CIC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:20Average %95
Range %89-103
1:40Average %90
Range %81-100
1:80Average %91
Range %86-99
1:160Average %95
Range %91-104
Recovery
The recovery of mouse CIC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-102
EDTA plasma (n=4)9690-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
10002.107 2.053 2.080 1.923
5001.593 1.575 1.584 1.427
2501.097 1.023 1.060 0.903
1250.635 0.611 0.623 0.466
62.50.449 0.426 0.438 0.281
31.20.302 0.283 0.293 0.136
15.60.226 0.217 0.222 0.065
00.152 0.162 0.157
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse CIC ELISA Kit was designed for the quantitative measurement of Mouse CIC protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.

Customer Reviews and Q&A

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 Q&A
Q:

Is this a C1q binding Elisa?

A:

There are generally antigen-specific and antigen-nonspecific methods for measuring CIC. The C1q binding ELISA belongs to the latter, but it is different from the method used by CSB-E16553m.

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