Target Name
low density lipoprotein cholesterol (LDL-C)
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 μg/mL-20 μg/mL
Detection Wavelength
450 nm
Research Area
Cardiovascular
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
86 |
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| Range % |
82-92 |
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| 1:200 |
Average % |
94 |
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| Range % |
91-98 |
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| 1:400 |
Average % |
107 |
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| Range % |
103-114 |
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| 1:800 |
Average % |
89 |
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| Range % |
85-94 |
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Recovery
| The recovery of mouse LDL-Cspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
88 |
84-93 |
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| EDTA plasma (n=4) |
91 |
87-95 |
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Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| μg/ml |
OD1 |
OD2 |
Average |
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| 20 |
0.105 |
0.107 |
0.106 |
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| 10 |
0.173 |
0.178 |
0.176 |
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| 5 |
0.248 |
0.255 |
0.252 |
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| 2.5 |
0.330 |
0.345 |
0.338 |
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| 1.25 |
0.430 |
0.443 |
0.437 |
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| 0.625 |
0.640 |
0.682 |
0.661 |
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| 0.312 |
0.941 |
1.011 |
0.976 |
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| 0 |
1.752 |
1.834 |
1.793 |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days
