Mouse low density lipoprotein cholesterol (LDL-C) ELISA kit

Code CSB-EQ027860MO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
low density lipoprotein cholesterol (LDL-C)
Abbreviation
LDL-C
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 μg/mL-20 μg/mL
Sensitivity
0.078 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:100 Average % 86    
Range % 82-92    
1:200 Average % 94    
Range % 91-98    
1:400 Average % 107    
Range % 103-114    
1:800 Average % 89    
Range % 85-94    
Recovery
The recovery of mouse LDL-Cspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 88 84-93    
EDTA plasma (n=4) 91 87-95    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
μg/ml OD1 OD2 Average      
20 0.105 0.107 0.106      
10 0.173 0.178 0.176      
5 0.248 0.255 0.252      
2.5 0.330 0.345 0.338      
1.25 0.430 0.443 0.437      
0.625 0.640 0.682 0.661      
0.312 0.941 1.011 0.976      
0 1.752 1.834 1.793      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

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