Mouse procollagen type III (HPCⅢ) ELISA Kit

Code CSB-E17125m
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation HPCⅢ
Target Name procollagen type III (HPCⅢ)
Alias procollagen type 3 (HPC3) ,procollagen type III (HPCⅢ)
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:100 Average % 97    
Range % 92-101    
1:200 Average % 95    
Range % 90-100    
1:400 Average % 91    
Range % 85-95    
1:800 Average % 102    
Range % 98-107    
Recovery
The recovery of mouse HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 89 85-93    
EDTA plasma (n=4) 84 80-88    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
1000 2.492 2.343 2.418 2.316    
500 1.825 1.716 1.771 1.669    
250 0.947 1.004 0.976 0.874    
125 0.571 0.582 0.577 0.475    
62.5 0.359 0.339 0.349 0.247    
31.2 0.246 0.237 0.242 0.140    
15.6 0.164 0.171 0.168 0.066    
0 0.098 0.105 0.102      
References
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