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Mouse procollagen type III (HPCⅢ) ELISA Kit

Mouse procollagen type III (HPCⅢ) ELISA Kit

Specification

Code

CSB-E17125m

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Lead Time

3-5 working days

Abbreviation

HPCⅢ

Target Name

procollagen type III (HPCⅢ)

Alias

procollagen type 3 (HPC3) ,procollagen type III (HPCⅢ)

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	97
	Range %	92-101
1:200	Average %	95
	Range %	90-100
1:400	Average %	91
	Range %	85-95
1:800	Average %	102
	Range %	98-107

Recovery

The recovery of mouse HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	84	80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.492	2.343	2.418	2.316
500	1.825	1.716	1.771	1.669
250	0.947	1.004	0.976	0.874
125	0.571	0.582	0.577	0.475
62.5	0.359	0.339	0.349	0.247
31.2	0.246	0.237	0.242	0.140
15.6	0.164	0.171	0.168	0.066
0	0.098	0.105	0.102

References

SIRT3 Facilitates Amniotic Fluid Stem Cells to Repair Diabetic Nephropathy Through Protecting Mitochondrial Homeostasis by Modulation of Mitophagy Feng J.et al,Cellular Physiology and Biochemistry,April 19

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Code	Product Name	Sample Types	Sensitivity
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Code	Product Name	Sample Types	Sensitivity
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Mouse procollagen type III (HPCⅢ) ELISA Kit

Specification

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