Product Details

Target Name

serum amyloid A2

Alternative Names

Saa2 ELISA Kit; Serum amyloid A-2 protein [Cleaved into: Amyloid protein A ELISA Kit; Amyloid fibril protein AA)] ELISA Kit

Abbreviation

SAA2

Uniprot No.

P05367

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

7.8 ng/mL-500 ng/mL

Sensitivity

1.95 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SAA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	108
	Range %	98-112
1:2	Average %	97
	Range %	88-101
1:4	Average %	90
	Range %	83-94
1:8	Average %	100
	Range %	90-104

Recovery

The recovery of mouse SAA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	93-106
EDTA plasma (n=4)	93	85-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
500	1.723	1.835	1.779	1.591
250	1.277	1.189	1.233	1.045
125	0.864	0.887	0.876	0.688
62.5	0.685	0.674	0.680	0.492
31.2	0.463	0.456	0.460	0.272
15.6	0.384	0.367	0.376	0.188
7.8	0.286	0.291	0.289	0.101
0	0.190	0.186	0.188

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse SAA2 ELISA Kit was designed for the quantitative measurement of Mouse SAA2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 ng/mL-500 ng/mL and the sensitivity is 1.95 ng/mL.

Target Background

Function
(From Uniprot)

Major acute phase reactant.

Gene References into Functions

High level SAA expression induced amyloidosis in all mice after a short, slightly variable delay. PMID: 23959890
These results suggest that the carboxy terminus of SAA, which is highly conserved among SAA sequences in all vertebrates, might play important structural roles, including modulating the folding, oligomerization, misfolding, and fibrillation of SAA.(Saa2) PMID: 22448726
The nonpathogenic murine Saa2.2 spontaneously forms marginally stable amyloid fibrils at 37 degrees C that exhibit cross-beta structure, binding to thioflavin T, and fibrillation by a nucleation-dependent seeding mechanism. PMID: 21942925
The ability of SAA2.2 to form different oligomeric species in vitro along with its marginal stability, suggest that the structure of SAA might be modulated in vivo to form different biologically relevant species. PMID: 21439938
forms a hexamer containing a central channel in solution PMID: 12456883
Only liposomes containing intact SAA2.1 or its residues 1-20 or 74-103 promoted the efflux of cholesterol in vivo PMID: 12951366
SAA2.2 can form amyloid fibrils in vitro at physiological temperatures, which suggests that SAA2.2's inability to cause amyloidosis may be related to the stabilization of hexameric SAA2.2 and/or the slow kinetics of aberrant misfolding and self-assembly. PMID: 16194868
Many functional and pathological roles attributed to serum amyloid A may rely on its precarious structure, modulated by its interaction with ligands under homeostasis conditions and during the acute phase response. PMID: 17425332

Hide All

Involvement in disease

Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function.

Subcellular Location

Secreted.

Protein Families

SAA family

Tissue Specificity

Expressed by the liver; secreted in plasma.

Database Links

KEGG: mmu:20209

STRING: 10090.ENSMUSP00000075365

UniGene: Mm.200941

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-EL020657MO
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Mouse serum amyloid A2 (SAA2) ELISA kit

Product Details

Related Products

Customer Reviews and Q&A

Target Background