Mouse serum amyloid A2 (SAA2) ELISA kit

Code CSB-EL020657MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name serum amyloid A2
Alternative Names Saa2 ELISA Kit; Serum amyloid A-2 protein [Cleaved into: Amyloid protein A ELISA Kit; Amyloid fibril protein AA)] ELISA Kit
Abbreviation SAA2
Uniprot No. P05367
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 7.8 ng/mL-500 ng/mL
Sensitivity 1.95 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SAA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 108
Range % 98-112
1:2 Average % 97
Range % 88-101
1:4 Average % 90
Range % 83-94
1:8 Average % 100
Range % 90-104
Recovery
The recovery of mouse SAA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 93-106
EDTA plasma (n=4) 93 85-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
500 1.723 1.835 1.779 1.591
250 1.277 1.189 1.233 1.045
125 0.864 0.887 0.876 0.688
62.5 0.685 0.674 0.680 0.492
31.2 0.463 0.456 0.460 0.272
15.6 0.384 0.367 0.376 0.188
7.8 0.286 0.291 0.289 0.101
0 0.190 0.186 0.188  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Major acute phase reactant.
Gene References into Functions
  1. High level SAA expression induced amyloidosis in all mice after a short, slightly variable delay. PMID: 23959890
  2. These results suggest that the carboxy terminus of SAA, which is highly conserved among SAA sequences in all vertebrates, might play important structural roles, including modulating the folding, oligomerization, misfolding, and fibrillation of SAA.(Saa2) PMID: 22448726
  3. The nonpathogenic murine Saa2.2 spontaneously forms marginally stable amyloid fibrils at 37 degrees C that exhibit cross-beta structure, binding to thioflavin T, and fibrillation by a nucleation-dependent seeding mechanism. PMID: 21942925
  4. The ability of SAA2.2 to form different oligomeric species in vitro along with its marginal stability, suggest that the structure of SAA might be modulated in vivo to form different biologically relevant species. PMID: 21439938
  5. forms a hexamer containing a central channel in solution PMID: 12456883
  6. Only liposomes containing intact SAA2.1 or its residues 1-20 or 74-103 promoted the efflux of cholesterol in vivo PMID: 12951366
  7. SAA2.2 can form amyloid fibrils in vitro at physiological temperatures, which suggests that SAA2.2's inability to cause amyloidosis may be related to the stabilization of hexameric SAA2.2 and/or the slow kinetics of aberrant misfolding and self-assembly. PMID: 16194868
  8. Many functional and pathological roles attributed to serum amyloid A may rely on its precarious structure, modulated by its interaction with ligands under homeostasis conditions and during the acute phase response. PMID: 17425332

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Involvement in disease Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function.
Subcellular Location Secreted.
Protein Families SAA family
Tissue Specificity Expressed by the liver; secreted in plasma.
Database Links

KEGG: mmu:20209

STRING: 10090.ENSMUSP00000075365

UniGene: Mm.200941

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