Mouse thyrotropin-releasing hormone,TRH ELISA KIT

Code CSB-E08147m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name thyrotropin-releasing hormone
Alternative Names Trh ELISA Kit; Pro-thyrotropin-releasing hormone ELISA Kit; Pro-TRH ELISA Kit; Prothyroliberin) [Cleaved into: Thyrotropin-releasing hormone ELISA Kit; TRH ELISA Kit; Protirelin ELISA Kit; TSH-releasing factor ELISA Kit; Thyroliberin ELISA Kit; Thyrotropin-releasing factor ELISA Kit; TRF)] ELISA Kit
Abbreviation TRH
Uniprot No. Q62361
Species Mus musculus (Mouse)
Sample Types serum
Detection Range 2.5 pg/mL-160 pg/mL
Sensitivity 0.625 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 85-94
1:2 Average % 105
Range % 91-113
1:4 Average % 94
Range % 90-98
1:8 Average % 85
Range % 80-89
The recovery of mouse TRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 91-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
160 2.604 2.475 2.540 2.421
80 1.482 1.408 1.445 1.326
40 0.917 0.969 0.943 0.824
20 0.621 0.643 0.632 0.513
10 0.375 0.386 0.381 0.262
5 0.296 0.281 0.289 0.170
2.5 0.168 0.172 0.170 0.051
0 0.121 0.117 0.119  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems.
Gene References into Functions
  1. co-regulation of the enzyme glutaminyl cyclase and its substrate TRH was reflected by a co-induction of both proteins in reactive astrocytes in proximity of Abeta deposits PMID: 25446989
  2. RXRA and RXRB display specific roles in modulating T3-dependent regulation of thyrotropin releasing hormone. PMID: 23896434
  3. Reversal of hypothyroidism in female patient after treatment with thyrotropin releasing hormone. PMID: 6432893
  4. Data suggest that expression of TRH is up-regulated by CREM (cAMP responsive element modulator protein) in hypothalamus; however, regulation of TRH by triiodothyronine appears independent of CREM or CREB (cAMP responsive element binding protein). PMID: 23000398
  5. Thyrotropin-releasing hormone altered the basal expression of the TSHbeta gene and the responsiveness of the gene to thyroid hormone. PMID: 22792320
  6. Klf4 plays a key role in the maturation of TRH expression in hypothalamic neurons. PMID: 21182892
  7. Data demonstrate that the distribution of hypophysiotropic TRH neurons in mice is vastly different from the pattern in rats, with a dominant occurrence of these neurosecretory cells in the compact part and adjacent regions at the mid-level of the PVN. PMID: 20737594
  8. These demonstrations of PPARgamma effects on hypothalamic Trh transcription in vivo consolidate the role of the TRH neuron as a central integrator of energy homeostasis. PMID: 19900503
  9. study provides functional in vivo data showing that both thyroid hormone receptor beta1 and beta2 isoforms have specific roles in regulating thyrotropin releasing hormone transcription PMID: 14726446
  10. Detailed analysis of TRH-knockout mice might provide insight into physiological significance of endogenous TRH. Present review focuses on phenotypic findings of mice deficient in TRH. PMID: 14751031
  11. Expression of thyrotorpin receptors 1 and 2, key elements in TRH regulation, was modified by thyroid status in the hypothalamus but not in the cortex. PMID: 15225659
  12. TRH is absolutely required for both TSH and TH synthesis but is not necessary for thyrotroph cell development PMID: 16339138
  13. TRH is not essential for pregnancy and lactation, but is required for full function of the lactotrophs, and the PRL mRNA level in the pituitary is regulated by TRH, both directly and indirectly via thyroid hormone PMID: 16484326
  14. Trh is expressed dynamically and specifically in the developing mouse definitive endoderm from E7.0 to E8.5 PMID: 17849455
  15. TRH neurons in the hypothalamic paraventricular nucleus suggest that CB1 endocannabinoids may influence both excitatory and inhibitory inputs of these neurons. PMID: 18818298
  16. provide evidence in mice that TSH participates in this photoperiodic signal transduction PMID: 19015516
  17. Results identify TRH as a modulator of orexin neuron firing in the hypothalamus, and suggest that orexin cell excitation may contribute to the arousal-enhancing actions of TRH. PMID: 19204048
  18. TRH modulates behavioral arousal, in part, through the Hcrt system. PMID: 19321767
  19. Direct receptor-mediated excitation of histaminergic tuberomamillary neurons by TRH demands activation of nonselective cation channels as well as electrogenic sodium/divalent calcium ion exchange. PMID: 19357273
  20. Released TRH is inactivated by an ectopeptidase, pyroglutamyl aminopeptidase II (PPII). Acute administration of alcohol modulates pyroglutamyl amino peptidase II activity and mRNA levels in rat limbic regions. PMID: 15707699
  21. There is considerable evidence linking alcohol consumption, sedation, and thyrotropin-releasing hormone (TRH) in the brain septum. PMID: 15240370

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Subcellular Location Secreted.
Protein Families TRH family
Tissue Specificity Specifically expressed in hypothalamus and testis.
Database Links

KEGG: mmu:22044

STRING: 10090.ENSMUSP00000006046

UniGene: Mm.1363


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