Product Details

Target Name

thyrotropin-releasing hormone

Alternative Names

Trh ELISA Kit; Pro-thyrotropin-releasing hormone ELISA Kit; Pro-TRH ELISA Kit; Prothyroliberin) [Cleaved into: Thyrotropin-releasing hormone ELISA Kit; TRH ELISA Kit; Protirelin ELISA Kit; TSH-releasing factor ELISA Kit; Thyroliberin ELISA Kit; Thyrotropin-releasing factor ELISA Kit; TRF)] ELISA Kit

Abbreviation

TRH

Uniprot No.

Q62361

Species

Mus musculus (Mouse)

Sample Types

serum

Detection Range

2.5 pg/mL-160 pg/mL

Sensitivity

0.625 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	105
	Range %	91-113
1:4	Average %	94
	Range %	90-98
1:8	Average %	85
	Range %	80-89

Recovery

The recovery of mouse TRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
160	2.604	2.475	2.540	2.421
80	1.482	1.408	1.445	1.326
40	0.917	0.969	0.943	0.824
20	0.621	0.643	0.632	0.513
10	0.375	0.386	0.381	0.262
5	0.296	0.281	0.289	0.170
2.5	0.168	0.172	0.170	0.051
0	0.121	0.117	0.119

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Mouse TRH ELISA Kit was designed for the quantitative measurement of Mouse TRH protein in serum. It is a Sandwich ELISA kit, its detection range is 2.5 pg/mL-160 pg/mL and the sensitivity is 0.625 pg/mL.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

I recently inquired about the TRH-ELISA kit through NacalaiTecque, Japan. I was informed that your TRH-ELISA kit only detects TRH and not pro-TRH. But this raises a new question: You provide Rat-TRH or human-TRH ELISA kits, how can these kits detect the differences between species? I don't think the TRH obtained as the final product varies from species to species.

We use different raw materials on the kits of different species. They have different characteristics as well as different matrix. As to the difference in detection results obtained from different species, we should take the result from the actual measurement as the real result.

Target Background

Function
(From Uniprot)

Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems.

Gene References into Functions

co-regulation of the enzyme glutaminyl cyclase and its substrate TRH was reflected by a co-induction of both proteins in reactive astrocytes in proximity of Abeta deposits PMID: 25446989
RXRA and RXRB display specific roles in modulating T3-dependent regulation of thyrotropin releasing hormone. PMID: 23896434
Reversal of hypothyroidism in female patient after treatment with thyrotropin releasing hormone. PMID: 6432893
Data suggest that expression of TRH is up-regulated by CREM (cAMP responsive element modulator protein) in hypothalamus; however, regulation of TRH by triiodothyronine appears independent of CREM or CREB (cAMP responsive element binding protein). PMID: 23000398
Thyrotropin-releasing hormone altered the basal expression of the TSHbeta gene and the responsiveness of the gene to thyroid hormone. PMID: 22792320
Klf4 plays a key role in the maturation of TRH expression in hypothalamic neurons. PMID: 21182892
Data demonstrate that the distribution of hypophysiotropic TRH neurons in mice is vastly different from the pattern in rats, with a dominant occurrence of these neurosecretory cells in the compact part and adjacent regions at the mid-level of the PVN. PMID: 20737594
These demonstrations of PPARgamma effects on hypothalamic Trh transcription in vivo consolidate the role of the TRH neuron as a central integrator of energy homeostasis. PMID: 19900503
study provides functional in vivo data showing that both thyroid hormone receptor beta1 and beta2 isoforms have specific roles in regulating thyrotropin releasing hormone transcription PMID: 14726446
Detailed analysis of TRH-knockout mice might provide insight into physiological significance of endogenous TRH. Present review focuses on phenotypic findings of mice deficient in TRH. PMID: 14751031
Expression of thyrotorpin receptors 1 and 2, key elements in TRH regulation, was modified by thyroid status in the hypothalamus but not in the cortex. PMID: 15225659
TRH is absolutely required for both TSH and TH synthesis but is not necessary for thyrotroph cell development PMID: 16339138
TRH is not essential for pregnancy and lactation, but is required for full function of the lactotrophs, and the PRL mRNA level in the pituitary is regulated by TRH, both directly and indirectly via thyroid hormone PMID: 16484326
Trh is expressed dynamically and specifically in the developing mouse definitive endoderm from E7.0 to E8.5 PMID: 17849455
TRH neurons in the hypothalamic paraventricular nucleus suggest that CB1 endocannabinoids may influence both excitatory and inhibitory inputs of these neurons. PMID: 18818298
provide evidence in mice that TSH participates in this photoperiodic signal transduction PMID: 19015516
Results identify TRH as a modulator of orexin neuron firing in the hypothalamus, and suggest that orexin cell excitation may contribute to the arousal-enhancing actions of TRH. PMID: 19204048
TRH modulates behavioral arousal, in part, through the Hcrt system. PMID: 19321767
Direct receptor-mediated excitation of histaminergic tuberomamillary neurons by TRH demands activation of nonselective cation channels as well as electrogenic sodium/divalent calcium ion exchange. PMID: 19357273
Released TRH is inactivated by an ectopeptidase, pyroglutamyl aminopeptidase II (PPII). Acute administration of alcohol modulates pyroglutamyl amino peptidase II activity and mRNA levels in rat limbic regions. PMID: 15707699
There is considerable evidence linking alcohol consumption, sedation, and thyrotropin-releasing hormone (TRH) in the brain septum. PMID: 15240370

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Subcellular Location

Secreted.

Protein Families

TRH family

Tissue Specificity

Specifically expressed in hypothalamus and testis.

Database Links

KEGG: mmu:22044

STRING: 10090.ENSMUSP00000006046

UniGene: Mm.1363

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Code	CSB-E08147m
Size	96T,5×96T,10×96T
Price	Request a Quote
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Mouse thyrotropin-releasing hormone,TRH ELISA KIT

Product Details

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Target Background