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Pig Alpha-fetoprotein(AFP) ELISA kit

Instructions
Code CSB-EL001421PI
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name alpha-fetoprotein
Alternative Names AFPAlpha-fetoprotein ELISA Kit; Alpha-1-fetoprotein ELISA Kit; Alpha-fetoglobulin ELISA Kit
Abbreviation AFP
Uniprot No. Q8MJ76
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %95
Range %88-102
1:2Average %92
Range %87-96
1:4Average %88
Range %82-94
1:8Average %97
Range %92-102
Recovery
The recovery of pig AFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8480-88
EDTA plasma (n=4)9186-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2002.416 2.352 2.384 2.289
1001.683 1.661 1.672 1.577
500.945 0.904 0.925 0.830
250.492 0.459 0.476 0.381
12.50.302 0.287 0.295 0.200
6.250.214 0.209 0.212 0.117
3.120.151 0.145 0.148 0.053
00.098 0.092 0.095 　
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin.
Subcellular Location Secreted
Protein Families ALB/AFP/VDB family
Tissue Specificity Plasma. Synthesized by the fetal liver and yolk sac.
Database Links

KEGG: ssc:397586

STRING: 9823.ENSSSCP00000009550

UniGene: Ssc.7131
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