Pig interleukin 17,IL-17 ELISA Kit

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Target Data

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Pig interleukin 17,IL-17 ELISA Kit

Name: Pig interleukin 17,IL-17 ELISA Kit
Brand: CUSABIO
SKU: CSB-E08057p

^Instructions

Code	CSB-E08057p
Size	96T,5×96T,10×96T
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Product Details
Citations
Related Products
Target Data

Target Name

interleukin 17A

Alternative Names

IL17A ELISA Kit; IL17 ELISA Kit; Interleukin-17A ELISA Kit; IL-17 ELISA Kit; IL-17A ELISA Kit

Abbreviation

IL17A

Uniprot No.

Q60I29

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, tissue homogenates

Detection Range

20 pg/mL- 800 pg/mL

Sensitivity

10 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-96
1:2	Average %	99
	Range %	92-106
1:4	Average %	89
	Range %	83-94
1:8	Average %	92
	Range %	87-98

Recovery

The recovery of pig IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-99
EDTA plasma (n=4)	84	80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
800	2.806	2.862	2.834
400	2.080	2.097	2.089
200	1.174	1.198	1.186
100	0.698	0.663	0.681
40	0.281	0.294	0.288
20	0.147	0.145	0.146

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Effects of GABA Supplementation on Intestinal SIgA Secretion and Gut Microbiota in the Healthy and ETEC-Infected Weanling Piglets Y Zhao,Mediators of Inflammation,2020

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Function	Ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
Gene References into Functions	At week 15, skin cell infiltrates from pigs with crusted scabies had significantly higher CD8+ T cell, gammadelta T cell and IL-17+ cell numbers than those with ordinary scabies. Peripheral IL-17 levels were not increased, suggesting that localized skin IL-17-secreting T cells may play a critical role in the pathogenesis of crusted scabies development. PMID: 25040151 Alterations in the IL-17 regulated pathway of porcine colon in response to dietary supplementation with seaweeds and yeast are reported. PMID: 23365350 conclude that only two subpopulations of porcine WBCs are sources of IL-17 after non-specific stimulation: CD3(+)CD4(+) and CD3(+)gammadeltaTCR(+). PMID: 22321808 Cloning and expression of interleukin-17. PMID: 15450131
Subcellular Location	Secreted
Protein Families	IL-17 family
Tissue Specificity	Highly expressed in adult heart, skin, and intestinal tissues, such as jejunum and ileum.
Database Links	KEGG: ssc:449530 STRING: 9823.ENSSSCP00000001905 UniGene: Ssc.42770