Pig interleukin 17,IL-17 ELISA Kit

Instructions
Code CSB-E08057p
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name interleukin 17A
Alternative Names IL17A ELISA Kit; IL17 ELISA Kit; Interleukin-17A ELISA Kit; IL-17 ELISA Kit; IL-17A ELISA Kit
Abbreviation IL17A
Uniprot No. Q60I29
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 20 pg/mL- 800 pg/mL
Sensitivity 10 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 86-96  
1:2 Average % 99  
Range % 92-106  
1:4 Average % 89  
Range % 83-94  
1:8 Average % 92  
Range % 87-98  
Recovery
The recovery of pig IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-99  
EDTA plasma (n=4) 84 80-88  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
800 2.806 2.862 2.834    
400 2.080 2.097 2.089    
200 1.174 1.198 1.186    
100 0.698 0.663 0.681    
40 0.281 0.294 0.288    
20 0.147 0.145 0.146    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
Gene References into Functions
  1. At week 15, skin cell infiltrates from pigs with crusted scabies had significantly higher CD8+ T cell, gammadelta T cell and IL-17+ cell numbers than those with ordinary scabies. Peripheral IL-17 levels were not increased, suggesting that localized skin IL-17-secreting T cells may play a critical role in the pathogenesis of crusted scabies development. PMID: 25040151
  2. Alterations in the IL-17 regulated pathway of porcine colon in response to dietary supplementation with seaweeds and yeast are reported. PMID: 23365350
  3. conclude that only two subpopulations of porcine WBCs are sources of IL-17 after non-specific stimulation: CD3(+)CD4(+) and CD3(+)gammadeltaTCR(+). PMID: 22321808
  4. Cloning and expression of interleukin-17. PMID: 15450131
Subcellular Location Secreted
Protein Families IL-17 family
Tissue Specificity Highly expressed in adult heart, skin, and intestinal tissues, such as jejunum and ileum.
Database Links

KEGG: ssc:449530

STRING: 9823.ENSSSCP00000001905

UniGene: Ssc.42770

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