Pig interleukin 17,IL-17 ELISA Kit

Code CSB-E08057p
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Pig IL17A ELISA Kit was designed for the quantitative measurement of Pig IL17A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 20 pg/mL- 800 pg/mL and the sensitivity is 10 pg/mL.

Target Name interleukin 17A
Alternative Names IL17A ELISA Kit; IL17 ELISA Kit; Interleukin-17A ELISA Kit; IL-17 ELISA Kit; IL-17A ELISA Kit
Abbreviation IL17A
Uniprot No. Q60I29
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 20 pg/mL- 800 pg/mL
Sensitivity 10 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 86-96  
1:2 Average % 99  
Range % 92-106  
1:4 Average % 89  
Range % 83-94  
1:8 Average % 92  
Range % 87-98  
Recovery
The recovery of pig IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-99  
EDTA plasma (n=4) 84 80-88  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
800 2.806 2.862 2.834    
400 2.080 2.097 2.089    
200 1.174 1.198 1.186    
100 0.698 0.663 0.681    
40 0.281 0.294 0.288    
20 0.147 0.145 0.146    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. Signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation. Plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. As a signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites. In airway epithelium, mediates neutrophil chemotaxis via induction of CXCL1 and CXCL5 chemokines. In secondary lymphoid organs, contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. Effector cytokine of a subset of gamma-delta T cells that functions as part of an inflammatory circuit downstream IL1B, TLR2 and IL23A-IL12B to promote neutrophil recruitment for efficient bacterial clearance. Effector cytokine of innate immune cells including invariant natural killer cell (iNKT) and group 3 innate lymphoid cells that mediate initial neutrophilic inflammation. Involved in the maintenance of the integrity of epithelial barriers during homeostasis and pathogen infection. Upon acute injury, has a direct role in epithelial barrier formation by regulating OCLN localization and tight junction biogenesis. As part of the mucosal immune response induced by commensal bacteria, enhances host's ability to resist pathogenic bacterial and fungal infections by promoting neutrophil recruitment and antimicrobial peptides release. In synergy with IL17F, mediates the production of antimicrobial beta-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, limiting the entry of microbes through the epithelial barriers. Involved in antiviral host defense through various mechanisms. Enhances immunity against West Nile virus by promoting T cell cytotoxicity. May play a beneficial role in influenza A virus (H5N1) infection by enhancing B cell recruitment and immune response in the lung. Contributes to influenza A virus (H1N1) clearance by driving the differentiation of B-1a B cells, providing for production of virus-specific IgM antibodies at first line of host defense.
Gene References into Functions
  1. At week 15, skin cell infiltrates from pigs with crusted scabies had significantly higher CD8+ T cell, gammadelta T cell and IL-17+ cell numbers than those with ordinary scabies. Peripheral IL-17 levels were not increased, suggesting that localized skin IL-17-secreting T cells may play a critical role in the pathogenesis of crusted scabies development. PMID: 25040151
  2. Alterations in the IL-17 regulated pathway of porcine colon in response to dietary supplementation with seaweeds and yeast are reported. PMID: 23365350
  3. conclude that only two subpopulations of porcine WBCs are sources of IL-17 after non-specific stimulation: CD3(+)CD4(+) and CD3(+)gammadeltaTCR(+). PMID: 22321808
  4. Cloning and expression of interleukin-17. PMID: 15450131
Subcellular Location Secreted.
Protein Families IL-17 family
Tissue Specificity Highly expressed in adult heart, skin, and intestinal tissues, such as jejunum and ileum.
Database Links

KEGG: ssc:449530

STRING: 9823.ENSSSCP00000001905

UniGene: Ssc.42770

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