Pig major acute phase protein (MAP) ELISA Kit

Pig major acute phase protein (MAP) ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Target Background

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

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Pig major acute phase protein (MAP) ELISA Kit

Citations (2) Protocol

Code	CSB-E13425p
Size	96T,5×96T,10×96T
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Product Details
Citations (2)
Related Products
Customer Reviews and Q&A
Target Background

Target Name

inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein)

Alternative Names

ITIH4 ELISA Kit; IHRP ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H4 ELISA Kit; ITI heavy chain H4 ELISA Kit; ITI-HC4 ELISA Kit; Inter-alpha-inhibitor heavy chain 4 ELISA Kit; Inter-alpha-trypsin inhibitor family heavy chain-related protein ELISA Kit; IHRP ELISA Kit; Major acute phase protein ELISA Kit; MAP ELISA Kit

Abbreviation

ITIH4

Uniprot No.

P79263

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, tissue homogenates

Detection Range

37.5 ng/mL-2400 ng/mL

Sensitivity

9.38 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig MAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	97
	Range %	92-104
1:400	Average %	95
	Range %	91-99
1:800	Average %	93
	Range %	87-99
1:1600	Average %	86
	Range %	82-92

Recovery

The recovery of pig MAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	97	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
2400	0.138	0.141	0.140
1200	0.457	0.460	0.459
600	0.903	0.898	0.901
300	1.134	1.121	1.128
150	1.518	1.483	1.501
75	1.689	1.706	1.698
37.5	2.032	2.049	2.041
0	2.187	2.114	2.151

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Pig ITIH4 ELISA Kit was designed for the quantitative measurement of Pig ITIH4 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 37.5 ng/mL-2400 ng/mL and the sensitivity is 9.38 ng/mL.

Hydroxy-Selenomethionine Supplementation During Gestation and Lactation Improve Reproduction of Sows by Enhancing the Antioxidant Capacity and Immunity Under Heat Stress Conditions J Wang, H Sun, Z Peng, SQ Wang, YQ Yan, WC Luo,Antioxidants,2025
Olive oil bioactives protect pigs against experimentally-induced chronic inflammation independently of alterations in gut microbiota Martin Liehr.et al,PLOS ONE,2017

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Function
(From Uniprot)

Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.

Gene References into Functions

Promoter of ITIH4 major acute phase protein has been isolated, cloned, and proven functional by response to an inflammatory stimulus (interleukin-6) in human Hep3B cells. (inter-alpha-trypsin heavy chain 4; ITIH4) PMID: 16275952

Subcellular Location

Secreted.

Protein Families

ITIH family

Tissue Specificity

Liver specific.

Database Links

KEGG: ssc:396799

STRING: 9823.ENSSSCP00000012206

UniGene: Ssc.50918

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ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.