Pig major acute phase protein (MAP) ELISA Kit

Code CSB-E13425p
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein)
Alternative Names
ITIH4 ELISA Kit; IHRP ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H4 ELISA Kit; ITI heavy chain H4 ELISA Kit; ITI-HC4 ELISA Kit; Inter-alpha-inhibitor heavy chain 4 ELISA Kit; Inter-alpha-trypsin inhibitor family heavy chain-related protein ELISA Kit; IHRP ELISA Kit; Major acute phase protein ELISA Kit; MAP ELISA Kit
Abbreviation
ITIH4
Uniprot No.
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
37.5 ng/mL-2400 ng/mL
Sensitivity
9.38 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig MAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 97  
Range % 92-104  
1:400 Average % 95  
Range % 91-99  
1:800 Average % 93  
Range % 87-99  
1:1600 Average % 86  
Range % 82-92  
Recovery
The recovery of pig MAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-101  
EDTA plasma (n=4) 97 90-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
2400 0.138 0.141 0.140    
1200 0.457 0.460 0.459    
600 0.903 0.898 0.901    
300 1.134 1.121 1.128    
150 1.518 1.483 1.501    
75 1.689 1.706 1.698    
37.5 2.032 2.049 2.041    
0 2.187 2.114 2.151    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig ITIH4 ELISA Kit was designed for the quantitative measurement of Pig ITIH4 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 37.5 ng/mL-2400 ng/mL and the sensitivity is 9.38 ng/mL.

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Target Background

Function
(From Uniprot)
Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.
Gene References into Functions
  1. Promoter of ITIH4 major acute phase protein has been isolated, cloned, and proven functional by response to an inflammatory stimulus (interleukin-6) in human Hep3B cells. (inter-alpha-trypsin heavy chain 4; ITIH4) PMID: 16275952
Subcellular Location
Secreted.
Protein Families
ITIH family
Tissue Specificity
Liver specific.
Database Links
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