Plant Ribulose-1,5-bisphosphate carboxylase/oxygenase(RuBisCO) ELISA Kit

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Code CSB-E16686Pl
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
Ribulose-1,5-bisphosphate carboxylase/oxygenase(RuBisCO)
Abbreviation
RuBisCO
Species
Plant (Mus musculus (Mouse)-Ear Cress)
Sample Types
plant tissues
Detection Range
3.12 μg/mL-800 μg/mL
Sensitivity
3.12 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<10%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<20%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Recovery
The recovery of plant RuBisCO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
leaf tissue (n=5) 95 89-103  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
800 0.047 0.050 0.049    
200 0.261 0.268 0.265    
50 0.467 0.473 0.470    
12.5 0.670 0.682 0.676    
3.12 1.139 1.143 1.141    
0 1.861 1.916 1.889    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Plant RuBisCO ELISA Kit was designed for the quantitative measurement of Plant RuBisCO protein in plant tissues. It is a Competitive ELISA kit, its detection range is 3.12 μg/mL-800 μg/mL and the sensitivity is 3.12 μg/mL.

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