Rabbit Aggrecan core protein(ACAN) ELISA kit

Code CSB-E12831Rb
Size 96T,5×96T,10×96T
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Product Details

Target Name
aggrecan 1
Alternative Names
ACAN ELISA Kit; AGC1 ELISA Kit; Aggrecan core protein ELISA Kit; Cartilage-specific proteoglycan core protein ELISA Kit; CSPCP ELISA Kit; Fragment ELISA Kit
Abbreviation
ACAN
Uniprot No.
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.625 ng/mL-40 ng/mL
Sensitivity
0.156 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ACAN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %98
Range %90-103
1:2Average %89
Range %82-94
1:4Average %90
Range %82-94
1:8Average %94
Range %87-98
Recovery
The recovery of rabbit ACAN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9084-94
EDTA plasma (n=4)10393-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
402.020 2.121 2.071 1.944
201.902 1.846 1.874 1.747
101.570 1.524 1.547 1.420
51.238 1.200 1.219 1.092
2.50.955 0.934 0.945 0.818
1.250.532 0.545 0.539 0.412
0.6250.315 0.309 0.312 0.185
00.128 0.126 0.127
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rabbit ACAN ELISA Kit was designed for the quantitative measurement of Rabbit ACAN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

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Target Background

Function
(From Uniprot)
This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.
Protein Families
Aggrecan/versican proteoglycan family
Database Links
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