Rabbit Sex hormone-binding globulin(SHBG) ELISA kit

Instructions
Code CSB-EL021252RB
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name sex hormone-binding globulin
Alternative Names SHBG ELISA Kit; Sex hormone-binding globulin ELISA Kit; SHBG ELISA Kit; Sex steroid-binding protein ELISA Kit; SBP ELISA Kit; Testis-specific androgen-binding protein ELISA Kit; ABP ELISA Kit
Abbreviation SHBG
Uniprot No. P15196
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 25 pmol/mL-400 pmol/mL
Sensitivity 25 pmol/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 90-103
1:2 Average % 89
Range % 83-99
1:4 Average % 95
Range % 90-101
1:8 Average % 89
Range % 85-100
Recovery
The recovery of rabbit SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 87-100
EDTA plasma (n=4) 95 89-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pmol/ml OD1 OD2 Average  
400 0.266 0.252 0.259  
200 0.528 0.541 0.535  
100 0.874 0.863 0.869  
50 1.348 1.325 1.337  
25 2.511 2.552 2.532  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration (By similarity).
Subcellular Location Secreted
Database Links

KEGG: ocu:100009224

STRING: 9986.ENSOCUP00000002218

UniGene: Ocu.2179

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