Rabbit terminal complement complex C5b-9 (TCC C5b-9)ELISA Kit

Code CSB-E15838Rb
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rabbit TCC C5b-9 ELISA Kit was designed for the quantitative measurement of Rabbit TCC C5b-9 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 7.8 ng/mL-500 ng/mL and the sensitivity is 1.95 ng/mL .

Target Name terminal complement complex C5b-9 (TCC C5b-9)
Abbreviation TCC C5b-9
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma
Detection Range 7.8 ng/mL-500 ng/mL
Sensitivity 1.95 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %80-92
1:2Average %96
Range %91-105
1:4Average %97
Range %92-103
1:8Average %91
Range %86-98
Recovery
The recovery of rabbit TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9789-109
EDTA plasma (n=4)9792-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
5002.659 2.563 2.611 2.519
2502.240 2.128 2.184 2.092
1251.488 1.375 1.432 1.340
62.50.845 0.817 0.831 0.739
31.20.444 0.434 0.439 0.347
15.60.247 0.239 0.243 0.151
7.80.156 0.148 0.152 0.060
00.093 0.091 0.092  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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