| Target Name |
terminal complement complex C5b-9 (TCC C5b-9) |
| Abbreviation |
TCC C5b-9 |
| Species |
Oryctolagus cuniculus (Rabbit) |
| Sample Types |
serum, plasma |
| Detection Range |
7.8 ng/mL-500 ng/mL |
| Sensitivity |
1.95 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | | |
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | | Sample | Serum(n=4) | | | 1:1 | Average % | 85 | | | Range % | 80-92 | | | 1:2 | Average % | 96 | | | Range % | 91-105 | | | 1:4 | Average % | 97 | | | Range % | 92-103 | | | 1:8 | Average % | 91 | | | Range % | 86-98 | |
|
| Recovery |
| The recovery of rabbit TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range | | | Serum (n=5) | 97 | 89-109 | | | EDTA plasma (n=4) | 97 | 92-108 | | | | | | | | | | | | | | | | | |
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.659 | 2.563 | 2.611 | 2.519 | | | 250 | 2.240 | 2.128 | 2.184 | 2.092 | | | 125 | 1.488 | 1.375 | 1.432 | 1.340 | | | 62.5 | 0.845 | 0.817 | 0.831 | 0.739 | | | 31.2 | 0.444 | 0.434 | 0.439 | 0.347 | | | 15.6 | 0.247 | 0.239 | 0.243 | 0.151 | | | 7.8 | 0.156 | 0.148 | 0.152 | 0.060 | | | 0 | 0.093 | 0.091 | 0.092 | | |
|
|
| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
