Rat β2-microglobulin,BMG/β2-MG ELISA Kit

Rat β2-microglobulin,BMG/β2-MG ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

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Product Details

Citations

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Customer Reviews and Q&A

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Rat β2-microglobulin,BMG/β2-MG ELISA Kit

Citations (3) Protocol MSDS

Code	CSB-E11298r
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (3)
Related Products
Customer Reviews and Q&A
Target Background

Target Name

beta-2-microglobulin

Alternative Names

B2MBeta-2-microglobulin ELISA kit

Abbreviation

B2M

Uniprot No.

P01885

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, urine, tissue homogenates

Detection Range

0.156 μg/mL-10 μg/mL

Sensitivity

0.039 μg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat BMG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	96
	Range %	92-101
1:4	Average %	100
	Range %	95-105
1:8	Average %	90
	Range %	85-95

Recovery

The recovery of rat BMG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-104
EDTA plasma (n=4)	93	89-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average	Corrected
10	2.645	2.678	2.662	2.515
5	1.855	1.896	1.876	1.729
2.5	1.256	1.263	1.260	1.113
1.25	0.834	0.869	0.852	0.705
0.625	0.487	0.489	0.488	0.341
0.312	0.325	0.353	0.339	0.192
0.156	0.261	0.284	0.273	0.126
0	0.145	0.149	0.147

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The Rat β2-microglobulin (BMG/β2-MG) ELISA Kit is your go-to solution for accurate and reliable quantification of Beta-2-microglobulin in rat serum, plasma, urine, and tissue homogenates.

Designed for researchers in the field of immunology、autoimmune diseases and renal function research, this ELISA kit provides a quantitative measurement of B2M levels with a detection range of 0.156 μg/mL-10 μg/mL and a sensitivity of 0.039 μg/mL, ensuring precise and reproducible results.

The assay principle is based on a sandwich measurement, where the captured rat β2-microglobulin is detected using a specific antibody conjugated to horseradish peroxidase. The kit's assay time is 1-5 hours, and the sample volume required is between 50-100ul.

The Rat β2-microglobulin (BMG/β2-MG) ELISA Kit is compatible with standard 96-well microplates and requires a detection wavelength of 450 nm. This ELISA kit has been cited in more than 3 scientific publications, demonstrating its robust performance and reliability.

MiR-122-5p and miR ‐326‐3p promote cadmium‐induced NRK‐52E cell apoptosis by downregulating PLD1 W Yuan,Environ. Toxicol,2020
Homoarginine supplementation improves blood glucose in diet-induced obese mice Stockebrand M. et al,Amino Acids. ,2015
Effect of salvianolic acid A and C compatibility on inflammatory cytokines in rats with unilateral ureteral obstruction. Li J.et al,J Tradit Chin Med.,2015

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Function
(From Uniprot)

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.

Subcellular Location

Secreted.

Protein Families

Beta-2-microglobulin family

Database Links

STRING: 9986.ENSOCUP00000014707

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Related pathways

Antigen processing and presentation

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.