Rat β2-microglobulin,BMG/β2-MG ELISA Kit

Code CSB-E11298r
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01885
Lead Time 3-5 working days
Abbreviation B2M
Protein Biological Process 1 Immunity
Target Name beta-2-microglobulin
Alias , beta chain of MHC class I molecules|beta-2-microglobin
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Immunity
Sample Types serum, plasma, urine, tissue homogenates
Detection Range 0.156 μg/mL-10 μg/mL
Sensitivity 0.039 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 914
RGD 2189
MGI 88127
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat BMG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %82-92
1:2Average %96
Range %92-101
1:4Average %100
Range %95-105
1:8Average %90
Range %85-95
Recovery
The recovery of rat BMG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9790-104
EDTA plasma (n=4)9389-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/mlOD1OD2AverageCorrected
102.645 2.678 2.662 2.515
51.855 1.896 1.876 1.729
2.51.256 1.263 1.260 1.113
1.250.834 0.869 0.852 0.705
0.6250.487 0.489 0.488 0.341
0.3120.325 0.353 0.339 0.192
0.1560.261 0.284 0.273 0.126
00.145 0.149 0.147  
References
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Function Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Subcellular Location Secreted
Protein Families Beta-2-microglobulin family
Database Links

STRING: 9986.ENSOCUP00000014707

Pathway Antigen processing and presentation

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