Code | CSB-EL001461RA |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of rat AGRN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 93 | ||||
Range % | 90-96 | |||||
1:2 | Average % | 84 | ||||
Range % | 80-88 | |||||
1:4 | Average % | 83 | ||||
Range % | 80-86 | |||||
1:8 | Average % | 89 | ||||
Range % | 86-91 |
The recovery of rat AGRN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 100 | 97-103 | ||||
EDTA plasma (n=4) | 96 | 93-99 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
The Rat Agrin (AGRN) ELISA kit is a reliable and accurate tool for measuring Agrin protein levels in samples from Rattus norvegicus (Rat). Agrin, encoded by the AGRN gene, is a protein that plays a crucial role in the development and maintenance of the neuromuscular junction.
This ELISA kit provides a simple and sensitive assay to quantify Rat Agrin in serum, plasma, and tissue homogenates with a detection range of 78 pg/mL to 5000 pg/mL and a sensitivity of 19.5 pg/mL. The assay can be completed in just 1-5 hours, making it an efficient and convenient tool for research purposes.
The Rat Agrin (AGRN) ELISA kit requires a sample volume of only 50-100 µl, minimizing the need for large sample sizes. Detection is performed at a wavelength of 450 nm, with results displayed in a standard curve format.
There are currently no reviews for this product.