Rat Agrin(AGRN) ELISA kit

Code CSB-EL001461RA
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Alternative Names
Agrn ELISA kit; AgrinAgrin [Cleaved into: Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment ELISA kit; C90); Agrin C-terminal 22 kDa fragment ELISA kit; C22)] ELISA kit
Abbreviation
AGRN
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
78 pg/mL-5000 pg/mL
Sensitivity
19.5 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat AGRN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 90-96  
1:2 Average % 84  
Range % 80-88  
1:4 Average % 83  
Range % 80-86  
1:8 Average % 89  
Range % 86-91  
Recovery
The recovery of rat AGRN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 100 97-103  
EDTA plasma (n=4) 96 93-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
5000 2.177 2.198 2.188 2.091  
2500 1.847 1.863 1.855 1.758  
1250 1.293 1.273 1.283 1.186  
625 0.811 0.846 0.829 0.732  
312.5 0.505 0.496 0.501 0.404  
156 0.301 0.321 0.311 0.214  
78 0.188 0.189 0.189 0.092  
0 0.096 0.098 0.097    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Rat Agrin (AGRN) ELISA kit is a reliable and accurate tool for measuring Agrin protein levels in samples from Rattus norvegicus (Rat). Agrin, encoded by the AGRN gene, is a protein that plays a crucial role in the development and maintenance of the neuromuscular junction.

This ELISA kit provides a simple and sensitive assay to quantify Rat Agrin in serum, plasma, and tissue homogenates with a detection range of 78 pg/mL to 5000 pg/mL and a sensitivity of 19.5 pg/mL. The assay can be completed in just 1-5 hours, making it an efficient and convenient tool for research purposes.

The Rat Agrin (AGRN) ELISA kit requires a sample volume of only 50-100 µl, minimizing the need for large sample sizes. Detection is performed at a wavelength of 450 nm, with results displayed in a standard curve format.

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Target Background

Function
(From Uniprot)
heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. This neuron-specific (z+) isoform is a component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses. This transmembrane agrin (TM-agrin) isoform, the predominate form in neurons of the brain, induces dendritic filopodia and synapse formation in mature hippocampal neurons in large part due to the attached glycosaminoglycan chains and the action of Rho-family GTPases.; Isoform 1, isoform 4, isoform 5 and isoform 6: neuron-specific (z+) isoforms that contain C-terminal insertions of 8-19 AA are potent activators of AChR clustering. Isoform 5, agrin (z+8), containing the 8-AA insert, forms a receptor complex in myotubules containing the neuronal AGRN, the muscle-specific kinase MUSK and LRP4, a member of the LDL receptor family. The splicing factors, NOVA1 and NOVA2, regulate AGRN splicing and production of the 'z' isoforms.; is involved in regulation of neurite outgrowth probably due to the presence of the glycosaminoglcan (GAG) side chains of heparan and chondroitin sulfate attached to the Ser/Thr- and Gly/Ser-rich regions. Also involved in modulation of growth factor signaling.; this released fragment is important for agrin signaling and to exert a maximal dendritic filopodia-inducing effect. All 'z' splice variants (z+) of this fragment also show an increase in the number of filopodia.
Gene References into Functions
  1. compared expression of agrin isoforms with the expression of AChE-T and ColQ in the developing spinal cord at the stages before and after the formation of NMJ basal lamina PMID: 23117006
  2. present the first crystal structure of an agrin-LRP4 complex which is essential for neuronal agrin-induced acetylcholine receptor (AChR) clustering PMID: 22302937
  3. agrin modulates neuropathic pain through NR1 phosphorylation in GABA neurons PMID: 21068333
  4. a model wherein the z8 insert is a Ca(2+)-responsive allosteric element that is essential in forming an active conformation in neuronal a PMID: 20566625
  5. Agrin binds BMP2, BMP4 and TGFbeta1 with relatively high affinity. PMID: 20505824
  6. different regions within the agrin protein are responsible for synapse formation at the neuromuscular junction and for process formation in central nervous system neurons PMID: 19940118
  7. The expression of agrin was studied during muscle development in a novel three-dimensional muscle-neuronal coculture system. PMID: 12955494
  8. Agrin deletion mutants show that axonal targeting of agrin depends on multiple domains that function in an additive fashion, including the very N-terminal portions and the C-terminal half of the molecule. PMID: 14697677
  9. Agrn becomes concentrated at neuroeffector and neuromuscular juncions in developing urinary bladder. PMID: 15711988
  10. Agrin may play a pivotal role in synaptogenesis in promoting a rapid switch between electrical coupling and synaptic neurotransmission. PMID: 15883200
  11. Agrin plays a role as a regulator of intercellular communication particularly junctional electric coupling and synapatic remodeling. PMID: 16274639
  12. Results suggest that by positively regulating filopodia in developing neurons, transmembrane agrin may influence the pattern of neurite outgrowth and synapse formation. PMID: 16860570
  13. Agrin, released from neurons, controls the maturation of the excitation-contraction coupling mechanism and due to modulation of RyRs and L-type Ca(2+) channels. A role for neural agrin in skeletal muscle maturation is proposed. PMID: 18003748
  14. Agrin plays an important role in the sprouting phase of reactive synaptogenesis; both its expression and distribution are correlated with extent of successful recovery after TBI. PMID: 18627255
  15. increasing the concentration of an ECM molecule, i.e. agrin, may enhance nicotine effects on pCREB PMID: 18639611
  16. transient post-ischemic hypothermia stabilizes brain vessels and reduces blood brain barrier disruption in part by preventing proteolytic degradation of regulatory basement membrane constituents, SPARC and agrin PMID: 19285050
  17. The results suggest a physiological role for Tm-agrin in the maturation of hippocampal neurons involving positive regulation of dendritic filopodia and promotion of synaptogenesis, but also suggest a role for axonal agrin in synaptogenesis. PMID: 19524020

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Subcellular Location
Cell junction, synapse. Cell membrane; Single-pass type II membrane protein.
Tissue Specificity
Embryonic nervous system and muscle.
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