Rat Agrin(AGRN) ELISA kit

Code CSB-EL001461RA
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P25304
Lead Time 7-14 working days
Abbreviation AGRN
Target Name agrin
Alias RP11-54O7.13, FLJ45064, agrin proteoglycan
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat AGRN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 90-96  
1:2 Average % 84  
Range % 80-88  
1:4 Average % 83  
Range % 80-86  
1:8 Average % 89  
Range % 86-91  
Recovery
The recovery of rat AGRN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 100 97-103  
EDTA plasma (n=4) 96 93-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
5000 2.177 2.198 2.188 2.091  
2500 1.847 1.863 1.855 1.758  
1250 1.293 1.273 1.283 1.186  
625 0.811 0.846 0.829 0.732  
312.5 0.505 0.496 0.501 0.404  
156 0.301 0.321 0.311 0.214  
78 0.188 0.189 0.189 0.092  
0 0.096 0.098 0.097    
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Function Isoform 1
Subcellular Location Cell junction, synapse, Cell membrane, Single-pass type II membrane protein
Tissue Specificity Embryonic nervous system and muscle.
Database Links

KEGG: rno:25592

STRING: 10116.ENSRNOP00000050623

UniGene: Rn.2163

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