Rat Chordin-like protein 1(CHRDL1) ELISA kit

Code CSB-EL005378RA
Size 96T,5×96T,10×96T
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Product Details

Target Name
chordin-like 1
Alternative Names
Chrdl1 ELISA Kit; Kjr ELISA Kit; Ng1Chordin-like protein 1 ELISA Kit; Kohjirin ELISA Kit; Neuralin-1 ELISA Kit; Neurogenesin-1 ELISA Kit; Ventroptin ELISA Kit
Abbreviation
CHRDL1
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.45 ng/mL-30 ng/mL
Sensitivity
0.11 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CHRDL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %81-96
1:2Average %96
Range %91-104
1:4Average %97
Range %92-105
1:8Average %95
Range %89-99
Recovery
The recovery of rat CHRDL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9894-108
EDTA plasma (n=4)9186-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
302.052 2.011 2.032 1.900
151.384 1.283 1.334 1.202
7.50.868 0.847 0.858 0.726
3.750.501 0.512 0.507 0.375
1.80.326 0.337 0.332 0.200
0.90.243 0.254 0.249 0.117
0.450.198 0.194 0.196 0.064
00.132 0.131 0.132  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat CHRDL1 ELISA Kit was designed for the quantitative measurement of Rat CHRDL1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.45 ng/mL-30 ng/mL and the sensitivity is 0.11 ng/mL .

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Target Background

Function
(From Uniprot)
Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embryonic bone formation. Plays a role during anterior segment eye development.
Gene References into Functions
  1. Ng1 gene could promote the proliferation of endogenous neural stem cells and protect injured neurons following spinal cord injury. PMID: 20459000
  2. Neurogenesin-1 contributes to neuronal differentiation of neural stem cells in the adult brain PMID: 14684875
Subcellular Location
Secreted.
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