Rat Collagen Type Ⅲ,Col Ⅲ ELISA KIT

Instructions
Code CSB-E07924r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Collagen Type Ⅲ,Col Ⅲ
Alternative Names Collagen Type 3,Col 3,Collagen Type III,Col III
Abbreviation Col Ⅲ
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 88-96  
1:2 Average % 98  
Range % 92-104  
1:4 Average % 95  
Range % 92-98  
1:8 Average % 86  
Range % 80-94  
Recovery
The recovery of rat Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-98  
EDTA plasma (n=4) 90 85-95  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.585 2.532 2.559 2.415  
5 1.795 1.719 1.757 1.613  
2.5 1.083 1.021 1.052 0.908  
1.25 0.657 0.705 0.681 0.537  
0.625 0.418 0.450 0.434 0.290  
0.312 0.299 0.314 0.307 0.163  
0.156 0.242 0.229 0.236 0.092  
0 0.146 0.141 0.144    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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