Rat Complement 4,C4 ELISA Kit

Instructions
Code CSB-E08706r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Complement 4,C4
Alternative Names C4 ELISA Kit; C4aComplement C4 [Cleaved into: Complement C4 beta chain; Complement C4 alpha chain; C4a anaphylatoxin; Complement C4 gamma chain] ELISA Kit
Abbreviation C4
Uniprot No. P08649
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 12.5 ng/mL-800 ng/mL
Sensitivity 3.12 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:200Average %99
Range %96-102
1:400Average %87
Range %82-93
1:800Average %100
Range %96-104
1:1600Average %84
Range %81-89
Recovery
The recovery of rat C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9390-96
EDTA plasma (n=4)8884-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
8002.604 2.658 2.631 2.540
4002.185 2.198 2.192 2.101
2001.673 1.698 1.686 1.595
1001.069 1.097 1.083 0.992
500.626 0.636 0.631 0.540
250.292 0.271 0.282 0.191
12.50.188 0.180 0.184 0.093
00.090 0.092 0.091
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes (By similarity).
Gene References into Functions
  1. Our study provides the first evidence that sustained selective EPAC activation leads to an increase in TRPC3 and C4 protein expression and induces a proarrhythmic SOCE-like activity in adult rat ventricular cardiomyocytes PMID: 26219954
  2. The expression of the complement component C3f and complement component 4A have potential for use in post-transplant diagnosis of rejection. PMID: 23613499
  3. This study presents the genomic organization of the two C4 gene-containing modules and the expression of the two C4 genes in the rat (Rattus norvegicus). PMID: 15634920
Subcellular Location Secreted, Cell junction, synapse, Cell projection, axon, Cell projection, dendrite
Database Links

KEGG: rno:24233

STRING: 10116.ENSRNOP00000037902

UniGene: Rn.155573

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