Product Details

Target Name

gastrin-releasing peptide

Alternative Names

GrpGastrin-releasing peptide ELISA Kit; GRP) [Cleaved into: Neuromedin-C ELISA Kit; GRP-10)] ELISA Kit

Abbreviation

GRP

Uniprot No.

P24393

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat GRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

Recovery

The recovery of rat GRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	92	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.654	2.526	2.590	2.404
500	2.147	2.048	2.098	1.912
250	1.446	1.562	1.504	1.318
125	0.982	0.996	0.989	0.803
62.5	0.568	0.551	0.560	0.374
31.2	0.372	0.361	0.367	0.181
15.6	0.278	0.274	0.276	0.090
0	0.186	0.185	0.186

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Rat GRP ELISA Kit was designed for the quantitative measurement of Rat GRP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Target Background

Function
(From Uniprot)

Stimulates the release of gastrin and other gastrointestinal hormones. Contributes to the perception of prurient stimuli and to the transmission of itch signals in the spinal cord that promote scratching behavior. Contributes primarily to nonhistaminergic itch sensation. Contributes to long-term fear memory, but not normal spatial memory. Contributes to the regulation of food intake.

Gene References into Functions

These results suggest that androgen surge during male puberty plays an important role in the development and maintenance of the male-specific GRP function in the rat spinal cord. PMID: 26860455
feeding responses evoked by the large molecular form of gastrin releasing peptide (GRP) in the rat PMID: 24993846
all forms of GRP reduce food intake in obese and lean adult Zucker rats similar to Bn in weanling rats. PMID: 24874706
The site of action of rat gastrin releasing peptide, in regulation of meal size and intermeal interval, is most likely the stomach and/or the duodenum. PMID: 24556509
Gastrin-releasing peptide terminals were found in spinal dorsal horn and trigeminal ganglion. PMID: 24254931
GRP regulation of meal size and intermeal interval occurs at different sites in the rat. PMID: 24291388
Gastrin-releasing peptide acts via postsynaptic bombesin type 2 receptors to modulate inward rectifier K+ and TRPV1-like conductances in rat paraventricular thalamic neurons PMID: 23359674
reduction of meal size and prolongation of intermeal interval (IMI) by GRP-29 require vagal and splanchnic nerves, and the duodenum is the major conduit that communicates prolongation of IMI by GRP-29 with the brain PMID: 22210008
With increasing levels of fear, levels of gastrin-releasing peptide (Grp) are increased in medial prefrontal cortex and basolateral amygdala. PMID: 20974156
GRP-29 and GRP-27 reduced the size of the first meal, prolonged intermeal interval and increased satiety ratio, but GRP-10 failed to exhibit similar feeding responses. The data support a role for GRP-29 in the short-term regulation of food intake. PMID: 21055429
These high-voltage electron microscopy findings provide an ultrastructural basis for understanding how the spinal GRP system regulates male sexual behavior. PMID: 19864484
Data suggest that GRP, by acting on GRP receptors but not via CRF receptors, increases 5-HT neuronal activity in the paraventricular nucleus. PMID: 12138009
GRP in the central part of the amygdala inhibits feeding, may decrease the efficiency of eating, and may act as a satiety signal. PMID: 12419521
the increase in gastrin relesing peptide synthesis in the neurosecretory structures of the hypothalamus shoud be regarded as a compensatory reaction in diabetes PMID: 12469881
GRP mRNA was detected in rat kidney from embryonic day 19 to postnatal day 30 by RT-PCR PMID: 15140764
Data suggest that GRP does affect the stress response. GRP alterations selectively affected fear-related responses. PMID: 17335915
Food deprivation and stimulation of the melanocortin system produce opposing changes in GRP gene expression in the PVN; GRP-containing neurons in the PVN may be part of the hypothalamic signaling pathway controlling food intake and energy balance. PMID: 18818295
the spinal GRP system appears to be a stress-vulnerable center for male reproductive functions PMID: 19169356
Results suggest that androgen signaling plays a pivotal role in the regulation of GRP expression in male lumbar spinal cord. PMID: 19359382

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Subcellular Location

Secreted. Cytoplasmic vesicle, secretory vesicle lumen.

Protein Families

Bombesin/neuromedin-B/ranatensin family

Database Links

KEGG: rno:171101

STRING: 10116.ENSRNOP00000022845

UniGene: Rn.10930

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Code	CSB-EL009940RA
Size	96T,5×96T,10×96T
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Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Rat Gastrin-releasing peptide(GRP) ELISA kit

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Target Background