Product Details

Target Name

insulin-like 3 (Leydig cell)

Alternative Names

Insl3 ELISA Kit; Rlf ELISA Kit; Insulin-like 3 ELISA Kit; Leydig insulin-like peptide ELISA Kit; Ley-I-L ELISA Kit; Relaxin-like factor) [Cleaved into: Insulin-like 3 B chain; Insulin-like 3 A chain] ELISA Kit

Abbreviation

INSL3

Uniprot No.

Q9WUK0

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat INSL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	105
	Range %	101-109
1:2	Average %	97
	Range %	94-99
1:4	Average %	88
	Range %	83-96
1:8	Average %	93
	Range %	89-98

Recovery

The recovery of rat INSL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	99-105
EDTA plasma (n=4)	92	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	1.978	1.944	1.961	1.759
5	1.436	1.545	1.491	1.289
2.5	1.021	1.006	1.014	0.812
1.25	0.665	0.645	0.655	0.453
0.625	0.423	0.412	0.418	0.216
0.312	0.312	0.325	0.319	0.117
0.156	0.255	0.270	0.263	0.061
0	0.198	0.205	0.202

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Rat INSL3 ELISA Kit was designed for the quantitative measurement of Rat INSL3 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Background

Function
(From Uniprot)

Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor.

Gene References into Functions

The observed postnatal difference in gubernacular malformations between two rate strains is due to different levels of the insl3 receptor differ among strains. PMID: 21633115
data suggest that INSL3 signaling in fetal gubernaculum induces morphogenetic programs in male genitalia; investigation of differences in gene expression regulation by INSL3 in gubernaculum of male and female genitalia PMID: 20631401
Demonstration of INSL3 as the ligand for LGR8 facilitates understanding of the mechanism of testis descent and allows studies on the role of INSL3 in gonadal and other physiological processes PMID: 12114498
Rat leucine-rich repeat-containing G protein-coupled receptor 8 bound INSL3 with high affinity, clearly demonstrating that it is the rat INSL3 receptor PMID: 15956681
INSL3/leucine-rich repeat-containing G protein-coupled receptor 8 signaling may be involved in the genesis and/or developmental maturation of renal glomeruli PMID: 15956754
Prenatal mono-n-butyl phthlate may inhibit the INSL3 gene expression associated with the transabdominal testicular ascent in fetal rats, thereby causing undescended testis in postnatal offspring PMID: 16338306
In utero exposure to flutamide decreases expression levels of Insl3 particularly at 15 days post conception. PMID: 18772241
INSL3 evidently traverses the blood-testis barrier to enter the seminiferous compartment, rete testis, and epididymis in sufficient concentration to be able to address the specific INSL3 receptors (RXFP2) on post-meiotic germ cells and in the epididymis. PMID: 19420383
Prenatal exposure to diaethylstilbestrol can inhibit transabdominal testicular descent via down-regulating the expression of INSL3, which is induced by down-regulating the expression of SF-1. PMID: 19493424

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Subcellular Location

Secreted.

Protein Families

Insulin family

Tissue Specificity

Expressed in Leydig cells of the testis, and weakly in the theca interna cells of antral follicles and the corpus luteum of the ovary.

Database Links

KEGG: rno:114215

STRING: 10116.ENSRNOP00000025349

UniGene: Rn.48700

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Code	CSB-E15850r
Size	96T,5×96T,10×96T
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Rat Insulin-Like 3 (INSL3) ELISA Kit

Product Details

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Target Background