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Rat Interleukin 12,IL-12/P40 ELISA Kit

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Protocol
Code CSB-E07362r-IS
Size 96T,5×96T,10×96T
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Product Details

Target Name
Interleukin 12,IL-12/P40
Abbreviation
IL-12/P40
Uniprot No.
Q9R278
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56pg/ml-100pg/ml
Sensitivity
0.78pg/ml
Assay Time
3.5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

　

Sample

Serum(n=4)

1:2

Average %

96

Range %

90-115

1:4

Average %

107

Range %

99-115

1:8

Average %

87

Range %

81-93

1:16

Average %

90

Range %

86-98

Recovery

The recovery of rat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

104

96-112

EDTA plasma (n=4)

97

90-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

100

3.648

3.582

3.615

3.491

50

3.086

2.909

2.998

2.874

25

2.394

2.296

2.345

2.221

12.5

1.696

1.707

1.701

1.578

6.25

0.939

0.902

0.920

0.797

3.12

0.560

0.564

0.562

0.438

1.56

0.323

0.332

0.327

0.204

0

0.124

0.123

0.124

　

ELISA Data Analysis
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Troubleshooting
and FAQs
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Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

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