Code | CSB-E13434r |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
To assess the linearity of the assay, samples were spiked with high concentrations of rat KISS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 85 | |||||
Range % | 82-88 | ||||||
1:2 | Average % | 91 | |||||
Range % | 87-96 | ||||||
1:4 | Average % | 105 | |||||
Range % | 101-109 | ||||||
1:8 | Average % | 96 | |||||
Range % | 93-99 |
The recovery of rat KISS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 87 | 84-90 | |||||
EDTA plasma (n=4) | 92 | 90-95 | |||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Rat KISS1 ELISA Kit was designed for the quantitative measurement of Rat KISS1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
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Please provide the following information regarding Rat Kisspeptin 1, KISS1 ELISA KitCSB-E13434r
a. What sample types have been tested with this kit.
b. What observed concentrations were obtained from these sample types and what dilutions were used?
c. Please provide the QC data for sample tested with this kit.
d. What is the standard? (Recombinant full-length protein? Partial protein (range?)?, peptide (sequence range?), native protein?)
e. If recombinant protein, then what is the host?
f. Is the capture antibody a monoclonal or polyclonal?
g. What is the host of the capture antibody?
h. Is the detection antibody a monoclonal or polyclonal?
i. What is the host of the detection antibody?