Rat Matrix Metalloproteinase 14,MMP-14 ELISA Kit

Code CSB-E13158r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
matrix metallopeptidase 14 (membrane-inserted)
Abbreviation
MMP14
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
46.88 pg/mL-3000 pg/mL
Sensitivity
11.75 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 91    
Range % 88-95    
1:2 Average % 95    
Range % 90-102    
1:4 Average % 97    
Range % 95-107    
1:8 Average % 101    
Range % 96-105    
Recovery
The recovery of rat MMP-14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 90 87-96    
EDTA plasma (n=4) 98 95-103    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
3000 2.288 2.289 2.289 2.092    
1500 1.736 1.752 1.744 1.547    
750 1.118 1.097 1.108 0.911    
375 0.674 0.727 0.701 0.504    
187.5 0.455 0.433 0.444 0.247    
93.75 0.336 0.331 0.334 0.137    
46.88 0.265 0.256 0.261 0.064    
0 0.205 0.189 0.197      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat MMP14 ELISA Kit was designed for the quantitative measurement of Rat MMP14 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 46.88 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL.

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Target Background

Function
(From Uniprot)
Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15 in association with pro-MMP2. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis.
Subcellular Location
Membrane; Single-pass type I membrane protein. Melanosome. Cytoplasm.
Protein Families
Peptidase M10A family
Database Links
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