Rat Neuromodulin(GAP43) ELISA kit

Instructions
Code CSB-EL009231RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name growth associated protein 43
Alternative Names Gap43 ELISA Kit; Neuromodulin ELISA Kit; Axonal membrane protein GAP-43 ELISA Kit; Growth-associated protein 43 ELISA Kit; Protein F1 ELISA Kit
Abbreviation GAP43
Uniprot No. P07936
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell lysates, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat GAP43 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %98
Range %90-102
1:2Average %102
Range %98-108
1:4Average %94
Range %88-99
1:8Average %97
Range %91-103
Recovery
The recovery of rat GAP43 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9894-102
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.107 2.276 2.192 2.037
101.513 1.616 1.565 1.410
50.955 0.923 0.939 0.784
2.50.546 0.557 0.552 0.397
1.250.389 0.372 0.381 0.226
0.6250.271 0.286 0.279 0.124
0.3120.191 0.198 0.195 0.040
00.154 0.156 0.155
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction (By similarity).
Gene References into Functions
  1. miR-204 promoted the apoptosis of retinal cells through inhibiting GAP-43 in optic nerve injury. PMID: 29286154
  2. The data indicate that endogenous Nrn1 and Gap43 mRNAs compete for binding to HuD for their axonal localization and activity of the Nrn1 3'UTR. PMID: 28871047
  3. Dexamethasone treatment significantly affects both GAP-43 and synaptophysin protein expression in the hippocampus of aged rats. PMID: 28801169
  4. Results show that repeated contingent, but not non-contingent, cocaine self-administration promotes an increase of nELAV expression immediately and 7 days after the last cocaine self-administration session, with a transient disappearance observed 24 h later. The evidence that the ubiquitous ELAV/HuR is not changed calls for a specific involvement of nELAV in these molecular mechanisms. PMID: 26850084
  5. Data show that growth-associated protein 43 (GAP43) gene-modified bone marrow-derived mesenchymal stem cells (BMSCs) transplantation can increase survival photoreceptor cells and delay retinal degeneration PMID: 27412933
  6. Data show that the paw skin and back skin revealed a significantly higher number of nerve fibres expressing growth-associated protein 43 (GAP-43) at both the acute and chronic stages of antigen-induced arthritis (AIA). PMID: 26503622
  7. astrocytic GAP43 upregulation may serve to indicate beneficial astrogliosis after central nervous system injury PMID: 26865625
  8. Lentiviral-mediated GAP-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats. PMID: 26238991
  9. Thus, unilateral deafness directly results in an asymmetrical adaptation of the gap43 transcription between both sides of the auditory brain stem. PMID: 25686598
  10. Phosphorylation at Ser-41 is required for plasma membrane association of GAP43. PMID: 25165142
  11. Studied whether melatonin would modulate the PSD-95, GAP-43, and MMP-9 proteins in cultured neurons exposed to glutamate excitotoxicity and in rats subjected to experimental stroke. PMID: 24350898
  12. The manifestation of clinical signs of experimental autoimmune encephalomyelitis has been found to be in correlation with the levels of GAP-43 proteolysis. PMID: 25868328
  13. Results show that PKC-dependent phosphorylation of GAP43 plays a critical role in regulating postsynaptic gephyrin aggregation in developing GABAergic synapses. PMID: 25755278
  14. Cocultivation with human adipose-derived mesenchymal stem cells restored GAP-43 expression and distribution in cortical neurons. PMID: 24458787
  15. There was no significant difference in immunohistochemical staining for transforming growth factor beta 3 between the control injured and obese injured groups PMID: 24665937
  16. KSRP modulation of GAP-43 mRNA stability restricts axonal outgrowth in embryonic hippocampal neurons. PMID: 24244461
  17. Progesterone promotes neuroprotection following traumatic brain injury by inhibiting the expression of Nogo-A and GFAP, and increasing GAP-43 expression. PMID: 24567055
  18. an AU-rich regulatory element (ARE) in GAP-43's 3'UTR is necessary and sufficient for axonal localization of Zbp1 PMID: 23586486
  19. Both GAP43 protein and mRNA levels were increased in dorsal root gangion neurons treated with neurotrophins and cocultured with skeletal muscle, implicating NTs and target skeletal muscle cells in DRG neuronal regeneration. PMID: 23666783
  20. Intradiscal inflammation might induce the expression of GAP-43, and potentially promote the nerve fiber ingrowth of rat. PMID: 23316631
  21. Ilexonin A markedly enhanced the neurogenesis in the brain after ischemia-reperfusion, and upregulated bFGF and GAP-43. PMID: 22121776
  22. Willed movement therapy increases the expression of NT-3 and GAP-43 in the ischemic brain area in rats with cerebral ischemia-reperfusion. PMID: 21868332
  23. siRNA-mediated depletion of overall GAP-43 mRNA from dorsal root ganglia decreases the length of axons. PMID: 23426659
  24. The mean brightness of GAP-43 per unit area of the of the dendritic tree does not vary across the estrous cycle; however, the modified brightness-area-product of GAP-43 changes significantly along the estrous cycle. PMID: 21948316
  25. Clonidine and dexmedetomidine treatment decreases the expression of GAP-43 in rat dorsal root ganglion during the development of chronic neuropathic pain. PMID: 22490962
  26. further evidence to support a key role for GAP-43 in brain development. PMID: 22617637
  27. IGF-1 may play an important role in neuroprotective effects on dorsal root ganglion neurons through regulating GAP-43 expression with excitotoxicity induced by Glu and the process was involved in both ERK1/2 and PI3K/Akt signaling pathways. PMID: 21822733
  28. Spinal GAP-43 is upregulated in the superficial L5 dorsal horn from 5 to 10 days after spinal cord injury; GAP-43/calcitonin-gene related peptide (CGRP) fibers may be indicative of sprouting peptidergic fibers. PMID: 21671799
  29. Noise-induced tinnitus is suppressed by strong up-regulation of GAP-43 in the medial ventral cochlear nucleus. PMID: 21821100
  30. This study suggested that GAP-43 liver change during function recovery after cerebellar damage. PMID: 22063813
  31. In PMCA-suppressed lines mRNA and protein levels of GAP43 were increased, however, the amount of phosphorylated form was lower than in control cells PMID: 21912933
  32. Exposure to low level lead during pregnancy could inhibit the GAP-43 protein and mRNA expression in the hippocampus of offspring. PMID: 18724891
  33. results show that the combination of repeated exposure to cocaine and acute stress significantly enhances nELAV expression and phosphorylation in the hippocampus with a concomitant increase of GAP43 expression PMID: 21210085
  34. BDNF and GAP43 may play an important role during the dynamic transhemispheric functional reorganization. PMID: 21723373
  35. GAP-43 is essential both to maintain climbing fibres structure in non-traumatic condition and to promote sprouting after partial lesion of the inferior olive. PMID: 21695168
  36. The expression of synaptophysin (SYP) and regenerationassociated protein (GAP43) in the injured brain of neural stem cells-transplanted rats was significantly increased. PMID: 21687946
  37. GFAP expression was down-regulated while GAP-43 expression upregulated in the retinal ganglial cells after peripheral nerve transplantation. PMID: 20423852
  38. Endogenous BDNF may regulate the expression of GAP-43 in the spinal cord anterior horn after sciatic nerve injury in rats. PMID: 20335141
  39. Significant correlations between gene and protein expressions were also observed for each transcript of BDNF, GAP-43, and NCAM1. PMID: 21436126
  40. Ttrahydroxystilbene glucoside can improve the neurological function through increasing the expressions of NGF and GAP-43 of cerebral ischemia-reperfusion rats. PMID: 21137370
  41. Data suggest that the expressions of GAP-43 mRNA and protein may be upregulated after brachial plexus injury, and GAP-43 protein is possibly associated with the axon regeneration and function reconstruction. PMID: 21167390
  42. results suggest that expression of GAP-43 in avulsed motoneurons is related to axonal regeneration whereas nNOS is not. PMID: 20667480
  43. Chondroitinase ABC can enhance the expression of GAP-43 after spinal cord injury. PMID: 21046809
  44. Study confirmed colocalization of autonomic nerve fiber markers with growth-associated protein 43 PMID: 20090303
  45. GAP-43 and P38 may be the pathological basis of insular epilepsy and the key molecular mechanism of synaptic plasticity. PMID: 20646586
  46. A role for pGAP-43 in retinotectal morphological plasticity observed both during normal development and after monocular enucleation is suggested. PMID: 20406666
  47. MMP-2, expressed and secreted by neurons and adjoining astrocytes, is a key actor in lesion-induced remodeling of central auditory networks and suggest a cooperativity with central axonal growth and synaptogenesis directed by GAP-43 PMID: 20173666
  48. study demonstrated that repeated maternal restraint stress increased GAP-43 in the postnatal rat brain during the second week of life but decreased after that PMID: 19782125
  49. Prenatal stress causes a significant increase of GAP-43 and pGAP-43 in the pup's hippocampus, which may alter the pattern of axonal growth and synapses' formation in the pup's brain PMID: 20035832
  50. Results provide marker proteins Gap-43 and newly identified candidate neuronal growth-associated proteins MAP1B, CRMP5, crmp5 and catenins. PMID: 19805073

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Subcellular Location Cell membrane, Peripheral membrane protein, Cytoplasmic side, Cell projection, growth cone membrane, Peripheral membrane protein, Cytoplasmic side, Cell junction, synapse, Cell projection, filopodium membrane, Peripheral membrane protein
Protein Families Neuromodulin family
Database Links

KEGG: rno:29423

STRING: 10116.ENSRNOP00000002091

UniGene: Rn.10928

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