Rat Neurotrophin 4,NT-4 ELISA KIT

Code CSB-E04691r
Size 96T,5×96T,10×96T
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Product Details

Target Name
neurotrophin 4
Alternative Names
Ntf4 ELISA Kit; Nt4 ELISA Kit; Ntf5 ELISA Kit; Neurotrophin-4 ELISA Kit; NT-4 ELISA Kit; Neurotrophin-5 ELISA Kit; NT-5 ELISA Kit; Neutrophic factor 4 ELISA Kit
Abbreviation
NTF4
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
47 pg/mL-3000 pg/mL
Sensitivity
11.75 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %86
Range %82-94
1:2Average %93
Range %88-98
1:4Average %96
Range %90-102
1:8Average %99
Range %95-104
Recovery
The recovery of rat NT-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9690-102
EDTA plasma (n=4)10095-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
30002.268 2.250 2.259 2.150
15001.845 1.871 1.858 1.749
7501.464 1.492 1.478 1.369
3751.135 1.149 1.142 1.033
187.50.704 0.724 0.714 0.605
940.438 0.457 0.448 0.339
470.287 0.294 0.291 0.182
00.108 0.110 0.109  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat NTF4 ELISA Kit was designed for the quantitative measurement of Rat NTF4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL .

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Target Background

Function
(From Uniprot)
Could serve as a target-derived trophic factor for sensory and sympathetic neurons.
Gene References into Functions
  1. The neuroprotective effect of NT-4 on retinas cultured under diabetic stress correlates with suppression of PERK kinase and transcription factor CHOP. PMID: 21767604
  2. alteration of NT-4 protein levels and gene expression in brain stem, cerebellum and cerebral hemisphere in context of ecstasy dependence; results show chronic administration of ecstasy significantly increased NT-4 gene expression in these areas of brain PMID: 21273656
  3. NT-4, alone or when co-expressed with brain-derived neurotrophic factor, is processed within and secreted by the constitutive secretory pathway PMID: 12970359
  4. The results indicate that oleic acid behaves as a neurotrophic factor, inducing GAP-43 expression through a PKC-mediated mechanism that is not mediated by other neurotrophic factors but that is strongly synergized by NT-3 and NT-4/5. PMID: 14519521
  5. NT-4 significantly improved slow motor unit recovery, and provides a basis for therapies intended to aid the functional recovery of muscles after denervating injury. PMID: 14622146
  6. BDNF and NT-4 expression could be modulated by activity of spinal circuitry and further support putative involvement of the endogenous neurotrophins in mechanisms of spinal neuroplasticity. PMID: 15193526
  7. Transcellular induction of neuropeptide Y expression by NT4 and BDNF. PMID: 15703301
  8. NT-4 regulates proliferation and differentiation of the dental epithelium and promotes production of the enamel matrix PMID: 18045880
  9. Analysis of neurotrophin expression in dentate gyrus dosen't reveal an expression change of Ntf4 in a genetically hypertensive strain of Wistar rat. PMID: 18957307

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Subcellular Location
Secreted.
Protein Families
NGF-beta family
Tissue Specificity
Expressed in thymus, muscle, ovary, brain, heart, stomach and kidney. Expressed in both embryo and adult tissues.
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