Rat Pancreatic alpha-amylase(AMY2A) ELISA kit

Code CSB-EL001689RA
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Abbreviation
AMY2A
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 mIU/mL-200 mIU/mL
Sensitivity
0.78 mIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat AMY2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 100  
Range % 95-104  
1:2 Average % 88  
Range % 84-93  
1:4 Average % 90  
Range % 85-94  
1:8 Average % 99  
Range % 94-105  
Recovery
The recovery of rat AMY2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 92-101  
EDTA plasma (n=4) 92 88-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml OD1 OD2 Average Corrected  
200 2.627 2.574 2.601 2.497  
100 1.985 1.852 1.919 1.815  
50 1.104 1.126 1.115 1.011  
25 0.628 0.609 0.619 0.515  
12.5 0.360 0.381 0.371 0.267  
6.25 0.259 0.247 0.253 0.149  
3.12 0.181 0.174 0.178 0.074  
0 0.103 0.105 0.104    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat AMY2A ELISA Kit was designed for the quantitative measurement of Rat AMY2A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 mIU/mL-200 mIU/mL and the sensitivity is 0.78 mIU/mL.

Citations

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Gene References into Functions
  1. Comparative proteomic analysis revealed that amylase levels were decreased by twofold in pancreases from hyperlipidemic rats compared with control rats.Pancreatic amylase protein levels were also decreased in tissue from hyperlipidemic rats. PMID: 20981735
  2. analysis of how amylase secretion is proteoglycan-dependent in rat parotid acinar cells PMID: 17980695
Subcellular Location
Secreted, extracellular space.
Protein Families
Glycosyl hydrolase 13 family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1