Rat Paraoxonase 1 (PON1)ELISA Kit

Code CSB-E14340r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P55159
Lead Time 3-5 working days
Abbreviation PON1
Target Name paraoxonase 1
Alias ESA, MVCD5, PON, arylesterase B-type|esterase A|paraoxonase B-type|serum aryldiakylphosphatase
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 μU/mL-1000 μU/mL
Sensitivity 3.9 μU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat PON1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:200Average %91
Range %86-95
1:400Average %102
Range %97-107
1:800Average %91
Range %85-97
1:1600Average %97
Range %91-103
Recovery
The recovery of rat PON1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-98
EDTA plasma (n=4)9790-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μU/mlOD1OD2AverageCorrected
10002.733 2.799 2.766 2.616
5002.274 2.220 2.247 2.097
2501.718 1.708 1.713 1.563
1251.103 1.123 1.113 0.963
62.50.641 0.682 0.662 0.512
31.20.351 0.362 0.357 0.207
15.60.245 0.236 0.241 0.091
00.150 0.149 0.150
References
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Function Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.
Subcellular Location Secreted, extracellular space
Protein Families Paraoxonase family
Tissue Specificity Plasma. Associated with HDL.
Database Links

KEGG: rno:84024

STRING: 10116.ENSRNOP00000011823

UniGene: Rn.20732

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