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Rat Podocalyxin,PCX ELISA Kit

Citations (2) Protocol MSDS
Code CSB-E13946r
Size 96T,5×96T,10×96T
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Product Details

Target Name
podocalyxin-like
Alternative Names
Podxl ELISA Kit; Pclp1 ELISA Kit; Podocalyxin ELISA Kit; Podocalyxin-like protein 1 ELISA Kit; PC ELISA Kit; PCLP-1 ELISA Kit
Abbreviation
PODXL
Uniprot No.
Q9WTQ2
Species
Rattus norvegicus (Rat)
Sample Types
serum, urine, cell culture supernates
Detection Range
1.56 ng/mL-100 ng/mL
Sensitivity
0.39 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat PCX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %87
Range %81-93
1:2Average %97
Range %93-101
1:4Average %89
Range %85-93
1:8Average %98
Range %92-104
Recovery
The recovery of rat PCX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9185-97
EDTA plasma (n=4)8983-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1002.201 2.312 2.257 2.112
501.574 1.456 1.515 1.370
251.009 1.056 1.033 0.888
12.50.633 0.654 0.644 0.499
6.250.401 0.412 0.407 0.262
3.120.272 0.286 0.279 0.134
1.560.210 0.215 0.213 0.068
00.144 0.146 0.145
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

CUSABIO's rat podocalyxin (PCX) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat PCX in serum, urine, or cell culture supernates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of PCX in samples. The color intensity is measured at 450 nm via a microplate reader.

PCX, also called PODXL, is a type I membrane mucoprotein abundantly presented in the podocytes of kidney glomeruli where it plays an important role in maintaining the structural organization of the kidney glomeruli and normal plasma filtration. Dominant and recessive mutations in PODXL are linked to human kidney disease. Abnormal expression of PODXL has been implicated in a range of cancers. PODXL is upregulated on tumors with high metastatic index. The presence of PODXL is associated with poor outcomes, thus emerging as an important prognostic and theragnostic marker in several human cancers.

Citations

Related Products

Podxl Proteins

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Target Background

Function
(From Uniprot)
Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
Gene References into Functions
  1. podocalyxin has a role in defining the structure of the podocyte basal surface PMID: 15226400
  2. Podocalyxin might be associated with the malignant progression of astrocytic tumors, and be a useful prognostic marker for astrocytic tumors. PMID: 18639524
  3. Report ubiquitous yet distinct expression of podocalyxin on vascular surfaces in normal and tumor tissues in the rat. PMID: 19142011
Subcellular Location
Apical cell membrane. Cell projection, microvillus. Membrane raft. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Membrane; Single-pass type I membrane protein.
Protein Families
Podocalyxin family
Tissue Specificity
Glomerular epithelium cell (podocyte) (at protein level).
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