PODXL

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP518830HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) PODXL.

Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP518830HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) PODXL.

Western Blot
Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate
All lanes: PODXL antibody at 2.5µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa

Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: PODXL antibody at 2.5µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa

Western Blot
Positive WB detected in: Mouse brain tissue
All lanes: PODXL antibody at 0.43µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa

Western Blot
Positive WB detected in: Rat brain tissue, Rat heart tissue, Rat kidney tissue
All lanes: PODXL antibody at 1.28µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA134471A0m at dilution of 1:200

Immunohistochemistry of paraffin-embedded human melanoma using CSB-MA134471A0m at dilution of 1:200

Immunofluorescent analysis of Hela cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of PC-3 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of A549 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of Ntera-2 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Overlay histogram showing Hela cells stained with CSB-MA134471A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*10⁶cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IGg2b (10µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunoprecipitating PODXL in HEK293 whole cell lysate
Lane 1: Mouse control IgG (1µg) instead of CSB-MA134471A0m in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA134471A0m (8µg) + HEK293 whole cell lysate (500µg)
Lane 3: HEK293 whole cell lysate (10µg)