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Rat Pulmonary Surfactant-associated protein C (SP-C) ELISA kit

Protocol
Code CSB-E12631r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
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* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
surfactant protein C
Alternative Names
Sftpc ELISA Kit; Sftp2 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; SP-C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit
Abbreviation
SFTPC
Uniprot No.
P11685
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
4.69 pg/mL-300 pg/mL
Sensitivity
1.17 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
　 Sample Serum(n=4)    
1:1000 Average % 85    
Range % 80-90    
1:2000 Average % 104    
Range % 100-109    
1:4000 Average % 91    
Range % 85-96    
1:8000 Average % 96    
Range % 91-103    
Recovery
The recovery of rat SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 98 94-102    
EDTA plasma (n=4) 87 83-91    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
300 2.402 2.274 2.338 2.227    
150 1.939 2.051 1.995 1.884    
75 1.411 1.497 1.454 1.343    
37.5 0.939 0.904 0.922 0.811    
18.75 0.568 0.579 0.574 0.463    
9.38 0.355 0.382 0.369 0.258    
4.69 0.248 0.260 0.254 0.143    
0 0.114 0.108 0.111 　    
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat SFTPC ELISA Kit was designed for the quantitative measurement of Rat SFTPC protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.69 pg/mL-300 pg/mL and the sensitivity is 1.17 pg/mL.

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Target Background

Function
(From Uniprot)
Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
Gene References into Functions
  1. SP-C is present on the surface of exocytosed surfactant particles. Antibodies against SP-C stabilize the released particles when contacting the air-liquid interface, precluding their transformation and the formation of surface films. SP-C acts as an essential, preformed molecular key in the initial stages of surfactant unpacking and surface film formation. PMID: 27155084
  2. misfolded surfactant protein C has a role in endoplasmic reticulum stress in epithelial-mesenchymal transition of alveolar epithelial cells PMID: 21169555
  3. Results suggest that there is no primary deficiency of surfactant proteins A, B, or C in the nitrofen-induced rat model of congenital diaphragmatic hernia. PMID: 12904592
  4. surfactant protein C processing requires a type II transmembrane topology directed by juxtamembrane positively charged residues PMID: 12933801
  5. NO mediated the decrease in surfactant protein gene expression PMID: 15640287
  6. Data indicate that heparin can be stimulatory to surfactant protein B and C gene expression depending on concentration, degree of sulfation, and surrounding fibroblast growth factor environment. PMID: 16794256

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Subcellular Location
Secreted, extracellular space, surface film.
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