Rat Renalase/ Monoamine Oxidase-C(RNLS/ MAO-C)ELISA Kit

Code CSB-EL002965RA
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
chromosome 10 open reading frame 59
Alternative Names
Rnls ELISA Kit; Renalase ELISA Kit; EC 1.6.3.5 ELISA Kit; Monoamine oxidase-C ELISA Kit; MAO-C ELISA Kit; alpha-NAD(P)H oxidase/anomerase ELISA Kit
Abbreviation
C10orf59
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
62.5 ng/mL-4000 ng/mL
Sensitivity
15.6 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat RNLS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 88-96
1:2 Average % 106
Range % 101-113
1:4 Average % 94
Range % 90-98
1:8 Average % 89
Range % 84-93
Recovery
The recovery of rat RNLS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 87 82-91
EDTA plasma (n=4) 95 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
4000 2.204 2.132 2.168 1.972
2000 1.619 1.524 1.572 1.376
1000 1.002 0.948 0.975 0.779
500 0.628 0.663 0.646 0.450
250 0.430 0.407 0.419 0.223
125 0.295 0.281 0.288 0.092
62.5 0.241 0.250 0.246 0.050
0 0.193 0.199 0.196  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat C10orf59 ELISA Kit was designed for the quantitative measurement of Rat C10orf59 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 ng/mL-4000 ng/mL and the sensitivity is 15.6 ng/mL.

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Target Background

Function
(From Uniprot)
Catalyzes the oxidation of the less abundant 1,2-dihydro-beta-NAD(P) and 1,6-dihydro-beta-NAD(P) to form beta-NAD(P)(+). The enzyme hormone is secreted by the kidney, and circulates in blood and modulates cardiac function and systemic blood pressure. Lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis.
Gene References into Functions
  1. The brain and peripheral tissues renalase mRNA levels demonstrate opposite trends, which are obviously crucial for impaired regulation of blood pressure in spontaneously hypertensive rats. PMID: 24113803
  2. exercise differentially regulates renalase expression in skeletal muscle and kidney in rats PMID: 24366404
  3. Renalase deficiency occurs in salt-sensitive Dahl rats as they develop hypertension[review] PMID: 17565281
Subcellular Location
Secreted.
Protein Families
Renalase family
Database Links
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